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RecombinantRabMAb

Recombinant Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)

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Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
  • Flow Cytometry (Intracellular) - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
  • Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
  • Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23934-20] to Cannabinoid Receptor I - BSA and Azide free
  • Suitable for: WB, Flow Cyt (Intra), ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free
    See all Cannabinoid Receptor I primary antibodies

  • Description

    Rabbit monoclonal [EPR23934-20] to Cannabinoid Receptor I - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt (Intra), ICC/IFmore details
    Unsuitable for: IHC-Fr,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain and hippocampus tissue lysate; Mouse brain tissue lysate; Rat brain tissue lysate; SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate; NIH/3T3 (mouse embryonic fibroblast) whole cell lysate. ICC/IF: SH-SY5Y cells; mouse primary neuron; rat primary neuron. Flow Cyt (intra): SH-SY5Y cells.

  • General notes

    ab278508 is the carrier-free version of ab259323.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab278508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Application notes
Is unsuitable for IHC-Fr,IHC-P or IP.

Target

Images

  • Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
    Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
    All lanes : Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human hippocampus tissue lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Rat brain tissue lysate

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 53 kDa



    This data was developed using ab259323, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

     Exposure time: Lane 1: 3 minutes; Lanes 2-4: 70 seconds.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
    Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)

    This data was developed using ab259323, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y cells labelling Cannabinoid receptor 1 with ab259323 at 1/100 (5.44 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in SH-SY5Y cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
    Flow Cytometry (Intracellular) - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)

    This data was developed using ab259323, the same antibody clone in a different buffer formulation. 

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling Cannabinoid receptor 1 with ab259323 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
    Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
    All lanes : Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323) at 1/1000 dilution

    Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
    Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 53 kDa



    This data was developed using ab259323, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: Lane 1: 67 seconds; Lane 2: 3 minutes.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
    Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)

    This data was developed using ab259323, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Cannabinoid receptor 1 with ab259323 at 1/500 (1.088 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
    Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
    All lanes : Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse liver tissue lysate

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 53 kDa



    This data was developed using ab259323, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile observed is consistent with what has been described in the literature (PMID: 9655502 ).

    Negative control: mouse liver (PMID: 9655502).

    Exposure time: 70 seconds

  • Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
    Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)

    This data was developed using ab259323, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling Cannabinoid receptor 1 with ab259323 at 1/500 (1.088 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab278508? Please let us know so that we can cite the reference in this datasheet.

ab278508 has not yet been referenced specifically in any publications.

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