Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat astrocytes)
Gel Running Conditions
Reduced Denaturing (10% SDS Polyacrylamide gels)
Loading amount
50 µg
Treatment
ACEA- 0.1nm, 1nm, 10nm for 15 mins
Specification
Rat astrocytes
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 49 kDa
Additional data
Additional Notes
Nitrocellulose membrane,
Phosphotase inhibitor and protease inhibitor cocktails from santa cruz
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Dhanush Haspula
Verified customer
Submitted Jun 22 2015