Recombinant
RabMAb

Recombinant Anti-Cannabinoid Receptor I (phospho S316) antibody [EPR2223(N)] - BSA and Azide free (ab243927)

Overview

  • Product name

    Anti-Cannabinoid Receptor I (phospho S316) antibody [EPR2223(N)] - BSA and Azide free
    See all Cannabinoid Receptor I primary antibodies
  • Description

    Rabbit monoclonal [EPR2223(N)] to Cannabinoid Receptor I (phospho S316) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cannabinoid Receptor I aa 300-400 (phospho S316). The exact sequence is proprietary.
    Database link: P21554

  • Positive control

    • Human fetal brain lysate; SH-SY5Y cells.
  • General notes

    Ab243927 is the carrier-free version of ab186428. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab243927 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab243927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 46, 53 kDa (predicted molecular weight: 53 kDa).
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CANN6 antibody
    • Cannabinoid receptor 1 antibody
    • CB-R antibody
    • CB1 antibody
    • CB1A antibody
    • CB1K5 antibody
    • CB1R antibody
    • Central cannabinoid receptor antibody
    • CNR antibody
    • CNR1 antibody
    • CNR1_HUMAN antibody
    • OTTHUMP00000016838 antibody
    • OTTHUMP00000214579 antibody
    see all

Images

  • Flow cytometry analysis of Cannabinoid Receptor I (phospho S316) expression in 2% paraformaldehyde-fixed SH-SY5Y cells using ab186428 at 1/200 dilution (red) or a rabbit IgG isotype control (green), SH-SY5Y cells preincubated with Cannabinoid Receptor I (phospho S316) peptide (blue) or non-phosopho Cannabinoid Receptor I (phospho S316) peptide (orange). FITC-conjugated goat anti-rabbit IgG (1/150 dilution) was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186428)

References

ab243927 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab243927.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up