• Product name

    Anti-Cannabinoid Receptor II antibody
    See all Cannabinoid Receptor II primary antibodies
  • Description

    Rabbit polyclonal to Cannabinoid Receptor II
  • Host species

  • Tested applications

    Suitable for: ICC/IF, IP, IHC-Fr, Flow Cyt, IHC-P, ICC, WBmore details
  • Species reactivity

    Reacts with: Mouse, Hamster, Human, Non human primates
  • Immunogen

    Fusion protein corresponding to Human Cannabinoid Receptor II aa 1-33 (N terminal).

  • Positive control

    • Human spinal cord lysate IF/ICC: SKNSH cell line.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 0.1% BSA, 49% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    Cannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9-tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P / Q type calcium channels. CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina.
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab3560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 22387618
IHC-Fr 1/100.
Flow Cyt 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.


IHC-P 1/100.
ICC 1/100.
WB 1/250 - 1/2500. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).


  • Function

    Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis.
  • Tissue specificity

    Preferentially expressed in cells of the immune system with higher expression in B cells and NK cells (at protein level). Expressed in skin in suprabasal layers and hair follicles (at protein level). Highly expressed in tonsil and to a lower extent in spleen, peripheral blood mononuclear cells, and thymus. PubMed:14657172 could not detect expression in normal brain. Expressed in brain by perivascular microglial cells and dorsal root glanglion sensory neurons (at protein level).
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational

    Constitutively phosphorylated on Ser-352; phosphorylation increases cell internalization and desensitizes the receptor.
  • Cellular localization

    Cell membrane. Cell projection > dendrite. Perikaryon. Localizes to apical dendrite of pyramidal neurons.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cannabinoid receptor 2 antibody
    • Cannabinoid receptor 2 macrophage antibody
    • CB 2 antibody
    • CB-2 antibody
    • CB2 antibody
    • CNR2 antibody
    • CNR2_HUMAN antibody
    • CNRII antibody
    • CX 5 antibody
    • CX5 antibody
    • hCB2 antibody
    • testis-dominant CNR2 isoform CB2 antibody
    see all


  • ICC/IF image of ab3560 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3560, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Shi J  et al. AM1241 alleviates MPTP-induced Parkinson's disease and promotes the regeneration of DA neurons in PD mice. Oncotarget 8:67837-67850 (2017). IHC-P . Read more (PubMed: 28978077) »
  • Seleverstov O  et al. Maternal alcohol exposure during mid-pregnancy dilates fetal cerebral arteries via endocannabinoid receptors. Alcohol 61:51-61 (2017). Read more (PubMed: 28554529) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Human Recombinant protein (H00001269-P01 (Novusbio), CNR2 recombinant protein)
H00001269-P01 (Novusbio), CNR2 recombinant protein
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Miss. Carina Kozmus

Verified customer

Submitted Nov 25 2015

Flow Cytometry
Human Cell (PBMC)
Gating Strategy
Lymphocytes B (CD19+)
Cell harvesting/tissue preparation method: ficoll
Sample buffer: PBS 1%BSA 0.01%NaN3

Abcam user community

Verified customer

Submitted Oct 16 2015


Thank you for contacting us.

We have following CB1 and CB2 available. These are testes in flow cytometry and are fully guaranteed.

ab3558; https://www.abcam.com/Cannabinoid-Receptor-I-antibody-ab3558.html

ab3561; https://www.abcam.com/Cannabinoid-Receptor-II-antibody-ab3561.html

ab3560; https://www.abcam.com/Cannabinoid-Receptor-II-antibody-ab3560.html

Could you let me know which conjugated you would like for compatible secondary antibody?

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for your inquiry.

The best way to find isotype controls for an antibody is to start at our homepage www.abcam.com. Go to the yellow bar at the top of the page and move the mouse to “Products,” then click on “Isotype Controls”. The direct link is:


Then select the correct isotype control based on the host species as well as the isotype, sub-isotype, conjugation, and application of the primary antibody which you are using.

For example, if the primary antibody is a FITC-conjugated mouse IgG1 for use in flow cytometry, then you will need to choose a FITC-conjugated mouse IgG1 isotype control tested in flow cytometry.

Please note that most isotype controls are monoclonal. They are not suitable for use with polyclonal antibodies as these contain more than one IgG subclass. However, we do provide several polyclonal isotype controls for use in these circumstances.

I taken the information that you have provided me to determine that the best isotype control in this instance is ab37415 Rabbit IgG- Chip Grade. Should you decide to conjugate a fluorescent tag onto your antibodies however,it is advised that you use a different isotype control which is also conjugated to the same fluorescent label.

Please let me know if have any questions. I would be happy to assist you.

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This product has been successfully tested in IHC with frozen sections. We have not tested the product with paraffin sections (formalin-fixed). It may work, but I would definitely use an antigen retrieval step. We have protocols listed on our website under the help heading for antigen retreival and IHC and I would recommend starting with the head-mediated pressure cooker method. Yes ab15372 could be used as a positive control or Jurkat T-cell lysates (ab7899) make for good controls for this product also. If you have any additional questions, please contact us again.

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