Anti-Cannabinoid Receptor II antibody (ab3561)
- Datasheet
- References (30)
- Protocols
Overview
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Product nameAnti-Cannabinoid Receptor II antibody
See all Cannabinoid Receptor II primary antibodies -
DescriptionRabbit polyclonal to Cannabinoid Receptor II
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-Fr, WB, IHC-P, ICC, ICC/IF, Flow Cytmore details
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Species reactivityReacts with: Mouse, Rat, Human, Chinese hamster
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Immunogen
Fusion protein corresponding to Rat Cannabinoid Receptor II aa 1-32 (N terminal).
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Positive control
- AtT20 cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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Primary antibody notesCannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9-tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P / Q type calcium channels. CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab3561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| IHC-Fr | 1/500. | |
| WB | 1/50 - 1/500. Predicted molecular weight: 40 kDa. | |
| IHC-P | 1/10 - 1/100. | |
| ICC | 1/500. | |
| ICC/IF | 1/500 - 1/5000. | |
| Flow Cyt | Use at an assay dependent concentration. PubMed: 18178718 ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionHeterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis.
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Tissue specificityPreferentially expressed in cells of the immune system with higher expression in B cells and NK cells (at protein level). Expressed in skin in suprabasal layers and hair follicles (at protein level). Highly expressed in tonsil and to a lower extent in spleen, peripheral blood mononuclear cells, and thymus. PubMed:14657172 could not detect expression in normal brain. Expressed in brain by perivascular microglial cells and dorsal root glanglion sensory neurons (at protein level).
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Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
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Post-translational
modificationsConstitutively phosphorylated on Ser-352; phosphorylation increases cell internalization and desensitizes the receptor. -
Cellular localizationCell membrane. Cell projection > dendrite. Perikaryon. Localizes to apical dendrite of pyramidal neurons.
- Information by UniProt
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Database links
- Entrez Gene: 1269 Human
- Entrez Gene: 12802 Mouse
- Entrez Gene: 57302 Rat
- Omim: 605051 Human
- SwissProt: P34972 Human
- SwissProt: P47936 Mouse
- SwissProt: Q9QZN9 Rat
- Unigene: 73037 Human
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Alternative names
- Cannabinoid receptor 2 antibody
- Cannabinoid receptor 2 macrophage antibody
- CB 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human tonsil tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human skin tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tssiue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Western blot - Anti-Cannabinoid Receptor II antibody (ab3561)All lanes : Anti-Cannabinoid Receptor II antibody (ab3561) at 1/200 dilution
Lane 1 : HT29 cell lysate
Lane 2 : C6 cell lysate
Lane 3 : Rat colon cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor II antibody (ab3561)Image courtesy of an anonymous Abreview.ab3561 staining Cannabinoid Receptor II in murine BV2 cells by Immunocytochemistry/ Immunofluorescence. Cells were stimulated with 10 uM ADP for 15 minutes at 37°C, 5%CO2 then washed 3X with PBS and fixed with 2% formaldehyde for 15 minutes at room temperature. A blocking step was performed for 1 hour at room temperature with 5% FBS/PBS/0.01% Triton-X-100. ab3561 used at a 1/500 dilution for 1 hour at room temperature then washed 3X PBS. The secondary was an Alexa Fluor 488 conjugated antibody, used at a 1/250 for 1 hour at room temperature, washed 3X PBS then mounted in Vectashield containing DAPI. -
Flow Cytometry - Anti-Cannabinoid Receptor II antibody (ab3561)Image from Leonti M et al, Biochem Pharmacol. 2010 Jun 15;79(12):1815-26. Epub 2010 Mar 3, Fig 6. DOI 10.1016/j.bcp.2010.02.015Quantification of CB1 and CB2 receptor surface expression in primary Human and HaCaT keratinocytes. (A) FL-1 shows autofluorescence (black population), non-specific binding of secondary antibody (dark grey population) and specific immunofluorescence obtained with CB1 (ab3558) and CB2 (ab3561) receptor antibodies, respectively (light grey population). Histogram shows representative measurement (5000 cells counted). (B) Differences in CB receptor expression between primary and HaCaT keratinocytes, showing marked increased expression of CB2 in primary keratinocytes (geo mean corrected for non-specific binding of secondary Ab). Data show mean values ±SEM obtained with two human primary keratinocyte samples and HaCaT cells, each measured three times.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)This image is courtesy of an Abreview submitted by Ms Nancy Nutile-McMenemyab3561 at 1/5000 staining Rat spinal cord tissue sections by IHC-P. Following nerve trans-section, the animal was transcardially perfused with PBS followed by 4% formaldehyde (no post fixation) then frozen in OCT and stored at -80°C until required. 20µm sections were cut and stained with the antibody for 18 hours. A biotinylated goat anti-rabbit antibody was used as the secondary.
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Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor II antibody (ab3561)Immunocytochemistry/immunofluorescence analysis of AtT20 cells transfect with the rat CB2 gene labeling Cannabinoid Receptor II with ab3561.
Protocols
References
This product has been referenced in:
- Wang B et al. Restoration of osteogenic differentiation by overexpression of cannabinoid receptor 2 in bone marrow mesenchymal stem cells isolated from osteoporotic patients. Exp Ther Med 15:357-364 (2018). Read more (PubMed: 29250156) »
- Corrado A et al. Endocannabinoid receptor CB2R is significantly expressed in aspirin-exacerbated respiratory disease: a pilot study. Int Forum Allergy Rhinol N/A:N/A (2018). Read more (PubMed: 29897665) »
