Anti-Cannabinoid Receptor II antibody (ab3561)

Rabbit polyclonal Cannabinoid Receptor II antibody. Validated in WB, IHC, ICC, Flow Cyt, ICC/IF and tested in Mouse, Rat, Human, Chinese hamster. Cited in 30 publication(s).

Overview

  • Product name
    Anti-Cannabinoid Receptor II antibody
    See all Cannabinoid Receptor II primary antibodies
  • Description
    Rabbit polyclonal to Cannabinoid Receptor II
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, WB, IHC-P, ICC, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Chinese hamster
  • Immunogen

    Fusion protein corresponding to Rat Cannabinoid Receptor II aa 1-32 (N terminal).

  • Positive control
    • AtT20 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 0.1% BSA, 49% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Cannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9-tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P / Q type calcium channels. CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/500.
WB 1/50 - 1/500. Predicted molecular weight: 40 kDa.
IHC-P 1/10 - 1/100.
ICC 1/500.
ICC/IF 1/500 - 1/5000.
Flow Cyt Use at an assay dependent concentration. PubMed: 18178718

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis.
  • Tissue specificity
    Preferentially expressed in cells of the immune system with higher expression in B cells and NK cells (at protein level). Expressed in skin in suprabasal layers and hair follicles (at protein level). Highly expressed in tonsil and to a lower extent in spleen, peripheral blood mononuclear cells, and thymus. PubMed:14657172 could not detect expression in normal brain. Expressed in brain by perivascular microglial cells and dorsal root glanglion sensory neurons (at protein level).
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications
    Constitutively phosphorylated on Ser-352; phosphorylation increases cell internalization and desensitizes the receptor.
  • Cellular localization
    Cell membrane. Cell projection > dendrite. Perikaryon. Localizes to apical dendrite of pyramidal neurons.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cannabinoid receptor 2 antibody
    • Cannabinoid receptor 2 macrophage antibody
    • CB 2 antibody
    • CB-2 antibody
    • CB2 antibody
    • CNR2 antibody
    • CNR2_HUMAN antibody
    • CNRII antibody
    • CX 5 antibody
    • CX5 antibody
    • hCB2 antibody
    • testis-dominant CNR2 isoform CB2 antibody
    see all

Images

  • ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human tonsil tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human skin tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tssiue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • All lanes : Anti-Cannabinoid Receptor II antibody (ab3561) at 1/200 dilution

    Lane 1 : HT29 cell lysate
    Lane 2 : C6 cell lysate
    Lane 3 : Rat colon cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa

  • ab3561 staining Cannabinoid Receptor II in murine BV2 cells by Immunocytochemistry/ Immunofluorescence. Cells were stimulated with 10 uM ADP for 15 minutes at 37°C, 5%CO2 then washed 3X with PBS and fixed with 2% formaldehyde for 15 minutes at room temperature. A blocking step was performed for 1 hour at room temperature with 5% FBS/PBS/0.01% Triton-X-100. ab3561 used at a 1/500 dilution for 1 hour at room temperature then washed 3X PBS. The secondary was an Alexa Fluor 488 conjugated antibody, used at a 1/250 for 1 hour at room temperature, washed 3X PBS then mounted in Vectashield containing DAPI.
  • Quantification of CB1 and CB2 receptor surface expression in primary Human and HaCaT keratinocytes. (A) FL-1 shows autofluorescence (black population), non-specific binding of secondary antibody (dark grey population) and specific immunofluorescence obtained with CB1 (ab3558) and CB2 (ab3561) receptor antibodies, respectively (light grey population). Histogram shows representative measurement (5000 cells counted). (B) Differences in CB receptor expression between primary and HaCaT keratinocytes, showing marked increased expression of CB2 in primary keratinocytes (geo mean corrected for non-specific binding of secondary Ab). Data show mean values ±SEM obtained with two human primary keratinocyte samples and HaCaT cells, each measured three times.

  • ab3561 at 1/5000 staining Rat spinal cord tissue sections by IHC-P. Following nerve trans-section, the animal was transcardially perfused with PBS followed by 4% formaldehyde (no post fixation) then frozen in OCT and stored at -80°C until required. 20µm sections were cut and stained with the antibody for 18 hours. A biotinylated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • Immunocytochemistry/immunofluorescence analysis of AtT20 cells transfect with the rat CB2 gene labeling Cannabinoid Receptor II with ab3561.

References

This product has been referenced in:
  • Shen X  et al. Decreased Expression of Cannabinoid Receptors in the Eutopic and Ectopic Endometrium of Patients with Adenomyosis. Biomed Res Int 2019:5468954 (2019). Read more (PubMed: 30800671) »
  • Lin M  et al. Activation of cannabinoid 2 receptor relieves colonic hypermotility in a rat model of irritable bowel syndrome. Neurogastroenterol Motil N/A:e13555 (2019). Read more (PubMed: 30793435) »
See all 39 Publications for this product

Customer reviews and Q&As

Filter by Application

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1-10 of 16 Abreviews

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HT29 cell line (colon cancer epithelial cell line))
Permeabilization
Yes - Tween 0,25%
Specification
HT29 cell line (colon cancer epithelial cell line)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Methanol

Abcam user community

Verified customer

Submitted Oct 30 2015

Application
Western blot
Sample
Rat Tissue lysate - whole (bladder)
Loading amount
100 µg
Specification
bladder
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Dr. Eun-Young Park

Verified customer

Submitted Apr 09 2013

Application
Immunohistochemistry free floating
Sample
Meriones unguiculatus Tissue sections (Brain)
Specification
Brain

Abcam user community

Verified customer

Submitted Jul 15 2011

Application
Western blot
Sample
Human Recombinant protein (Brian)
Loading amount
10 µg
Specification
Brian
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 01 2011

Application
Western blot
Sample
Rat Cell lysate - whole cell (primary cortical microglia)
Loading amount
100000 cells
Specification
primary cortical microglia
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Protein-free Blocking Solution (Pierce) as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jan 18 2010

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (primary cortical microglia)
Specification
primary cortical microglia
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Oct 14 2009

Application
Western blot
Sample
Human Cell lysate - whole cell (DLD-1 cells)
Loading amount
40000 cells
Specification
DLD-1 cells
Gel Running Conditions
Reduced Denaturing (4-12% gel)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted May 28 2009

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Macrophage)
Specification
Macrophage
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Mar 26 2009

Application
Immunocytochemistry/ Immunofluorescence
Sample
Chinese Hamster Cell (recombinant expression of human CB2 receptor)
Specification
recombinant expression of human CB2 receptor
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Mar 26 2009

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (ear)
Specification
ear
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Based on a citric acid formula
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 18 2007

1-10 of 16 Abreviews

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