Application
Western blot
Sample
Rat Cell lysate - whole cell (primary cortical microglia)
Loading amount
100000 cells
Specification
primary cortical microglia
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Protein-free Blocking Solution (Pierce) as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Protein-free Blocking Solution (Pierce)
Secondary antibody
Name
Non-Abcam antibody was used: BioRad anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
SuperSignal West PICO kit
Exposure
7 minute(s) and 0 second(s)
Bands
Specific: 36 kDa Non-specific: many kDa
Additional data
Additional Notes
Laemmli Buffer was used as lysis buffer, no protease inhibitors were added.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Jan 18 2010