Overview

  • Product name
    Anti-Cannabinoid Receptor II antibody
    See all Cannabinoid Receptor II primary antibodies
  • Description
    Rabbit polyclonal to Cannabinoid Receptor II
  • Host species
    Rabbit
  • Specificity
    According to BLAST results, the antibody could cross-react with both rat isoforms. No experiments were done to confirm this possibility.
  • Tested applications
    Suitable for: IHC-FoFr, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Rat Cannabinoid Receptor II.

    Read Abcam's proprietary immunogen policy (Peptide available as ab45941.)

  • Positive control
    • ab45942 gave a positive result in the following tissue lysates: Rat Spinal Cord, and Mouse Thymus.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab45942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/300.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 45 kDa).Can be blocked with Cannabinoid Receptor II peptide (ab45941).
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis.
  • Tissue specificity
    Preferentially expressed in cells of the immune system with higher expression in B cells and NK cells (at protein level). Expressed in skin in suprabasal layers and hair follicles (at protein level). Highly expressed in tonsil and to a lower extent in spleen, peripheral blood mononuclear cells, and thymus. PubMed:14657172 could not detect expression in normal brain. Expressed in brain by perivascular microglial cells and dorsal root glanglion sensory neurons (at protein level).
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications
    Constitutively phosphorylated on Ser-352; phosphorylation increases cell internalization and desensitizes the receptor.
  • Cellular localization
    Cell membrane. Cell projection > dendrite. Perikaryon. Localizes to apical dendrite of pyramidal neurons.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cannabinoid receptor 2 antibody
    • Cannabinoid receptor 2 macrophage antibody
    • CB 2 antibody
    • CB-2 antibody
    • CB2 antibody
    • CNR2 antibody
    • CNR2_HUMAN antibody
    • CNRII antibody
    • CX 5 antibody
    • CX5 antibody
    • hCB2 antibody
    • testis-dominant CNR2 isoform CB2 antibody
    see all

Images

  • ab45942 staining Cannabinoid Receptor II in Rat ileum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Bouin's solution and blocked with 10% BSA for 10 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody for 17 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

  • Immunocytochemical immunofluorescence analysis of methanol-fixed HT29 human cell line, labelling cannabinoid receptor II with ab45942 at 1/50 dilution incubated for 18 hours at 4°C in 1% BSA. Fixed cells were permeabilized with 0.25% Tween. Secondary used was a Goat anti-Rabbit polyclonal Alexa Fluor® 488. Counterstain is DAPI against nuclear DNA.

    See Abreview

  • All lanes : Anti-Cannabinoid Receptor II antibody (ab45942) at 1 µg/ml

    Lane 1 : Rat spinal cord tissue lysate
    Lane 2 : Mouse thymus tissue lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 8 minutes
  • ICC/IF image of ab45942 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45942, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Miragliotta V  et al. Cannabinoid receptor types 1 and 2 and peroxisome proliferator-activated receptor-a: distribution in the skin of clinically healthy cats and cats with hypersensitivity dermatitis. Vet Dermatol N/A:N/A (2018). Read more (PubMed: 29920828) »
  • Elbaz M  et al. Novel role of cannabinoid receptor 2 in inhibiting EGF/EGFR and IGF-I/IGF-IR pathways in breast cancer. Oncotarget 8:29668-29678 (2017). Read more (PubMed: 27213582) »
See all 8 Publications for this product

Customer reviews and Q&As

1-10 of 12 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Cat Tissue sections (Gastrointestinal tract)
Permeabilization
Yes - Triton
Specification
Gastrointestinal tract
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 20°C
Fixative
Paraformaldehyde

Chiocchetti Roberto

Verified customer

Submitted Dec 04 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (ileum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - Triton x 100
Specification
ileum
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Bouin's solution

Dr. Mehmet Özbek

Verified customer

Submitted Jul 25 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (fetus, ileum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - Triton x 100
Specification
fetus, ileum
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Bouin's solution

Dr. Mehmet Özbek

Verified customer

Submitted Jul 25 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (ileum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - Triton x 100
Specification
ileum
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Bouin's solution

Dr. Mehmet Özbek

Verified customer

Submitted Jul 25 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HT29 cell line (colon cancer epithelial cell line))
Permeabilization
Yes - Tween 0,25%
Specification
HT29 cell line (colon cancer epithelial cell line)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Methanol

Abcam user community

Verified customer

Submitted Oct 30 2015

Answer

Vielen Dank für Ihre Antwort.

Die Referenznummer Ihrer Gutschrift ist xxxxx.

Es tut mir leid, dass der Antikörper nicht so funktioniert hat, wie auf dem Datenblatt angegeben. Ich habe unsere Finanzabteilung beauftragt, eine Gutschrift für Sie auszustellen. Sie können diese Gutschrift auf folgende Weise verwenden:

(1) Gegen die Rechnung dieses Antikörpers verwenden falls diese noch nicht bezahlt wurde
(2) Aufbewahren und gegen eine zukünftige Rechnung verwenden
(3) Wir stellen Ihnen gerne eine komplette Rückerstattung aus, wenn keine offenen Rechnungen mehr ausstehen

Um eine Rückerstattung des Kaufpreises zu erhalten oder um zu bestätigen wie Sie diese Gutschrift benutzen möchten, möchte ich Sie bitten, sich mit unserer Finanzabteilung in Verbindung zu setzen.
Sie können unsere Finanzabteilung per Email unter der Adresse creditcontrol@abcam.com oder telefonisch unter der Nummer 030 896 779 154 erreichen.

Bitte geben Sie die Referenznummer Ihrer Gutschrift immer mit an.

Die Referenznummer ist für Ihre Unterlagen bestimmt. Wir schicken Ihnen die eigentliche Gutschrift per Post oder Email zu. Sie enthält auch die Gutschriftnummer, die mit dem Buchstaben CGB beginnt.

Ich hoffe dass Sie trotz dieser schlechten Erfahrung weiterhin Kunde bei uns bleiben. Bitte zögern Sie nicht sich wieder an uns zu wenden, falls Sie weitere Fragen haben.

Read More

Answer

Vielen Dank für Ihre Antwort.

Es tut mir leid, dass Sie diese Probleme mit unserem Antikörper hatten. Ich habe mir die Protokollangaben noch einmal angeschaut. Leider gibt es nicht viele Vorschläge die wir machen könnten um das Resultat zu verbessern.

Da der Antikörper für WB mit Maus Proben garantiert ist würde ich gerne als Kompensation eine Gutschrift ausstellen oder Ihnen ein Ersatzprodukt zukommen lassen.

Für einen nächsten Versuch würde ich gerne ein paar Sachen anmerken die in Erwägung gezogen werden könnten.

1. Wir empfehlen mit einer Antikörperverdünnung von 1:500 zu starten. Sollte das detektierte Signal zu stark sein empfehlen wir eine höhere Verdünnung mit dem nachfolgenden Versuch.

2. Für manche Antikörper ist die Wahl des blocking Reagents entscheidend. Wir würden empfehlen zu versuchen mit 5% BSA zu blocken. Ein Beispiel in wie weit die verschiedenen blocking Reagenzien einen Unterschied machen können Sie hier einsehen:

https://www.abcam.com/ab9385

3. Es wäre hilfreich eine positiv Kontrolle für den jeweiligen Antikörper mit dem nächsten Versuch zu machen. Mit der positiv Kontrolle kann die Qualität des Antikörpers sicher bestimmt werden. Eine passende positiv Kontrolle für den Anti-Cannabinoid Receptor II antibody wäre zum Beispiel Human Spinal Cord, Rat Spinal Cord und Mouse Thymus.

Ich hoffe diese Informationen sind hilfreich für Sie und freue mich auf eine Antwort wie Sie in diesem Fall weiter verfahren möchten.

Read More

Answer

Thank you for your enquiry. I am sorry to hear ab23703 and ab45942 are giving you trouble.So I assist you more efficiently, please complete the questions below in your reply.

Thanks in advance for the additional information!

+++

It will take approximately 5 minutes to complete the questionnaire. Please fill-in all fields so that we can better assist you.



1) Abcam product code ab



2) Abcam order reference number or product batch number



3) Description of the problem



4) Sample preparation:

Type of sample (whole cell lysates, fraction, recombinant protein…):

Lysis buffer

Protease inhibitors:

Phosphatase inhibitors

Reducing agent

Boiling for ≥5 min? yes/no

Protein loaded ug/lane or cells/lane

Positive control

Negative control



5) Percentage of gel

Type of membrane

Protein transfer verified

Blocking agent and concentration

Blocking time

Blocking temperature



6) Primary antibody (If more than one was used, describe in “additional notes”) :

Concentration or dilution

Diluent buffer

Incubation time

Incubation temperature:



7) Secondary antibody:

Species:

Reacts against:

Concentration or dilution

Diluent buffer

Incubation time

Incubation temperature:

Fluorochrome or enzyme conjugate:



8) Washing after primary and secondary antibodies:

Buffer

Number of washes



9)Detection method

10) How many times have you run this staining?

Do you obtain the same results every time?

What steps have you altered to try and optimize the use of this antibody?



Document attachment: Attaching images of your blot is strongly recommended and can greatly speed up our investigation of your problem.





Please send this questionnaire by e-mail by cutting and pasting the text in your e-mail (no attachments other than images) to technical@abcam.com.

Read More
Application
Western blot
Sample
Human Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
Brain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 01 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Brain)
Permeabilization
Yes - 0.3% Triton
Specification
Brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 25 2011

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up