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Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Rat Cannabinoid Receptor II.
(Peptide available as ab45941.)
Our Abpromise guarantee covers the use of ab45942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 45 kDa).Can be blocked with Cannabinoid Receptor II peptide (ab45941).|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 5 µg/ml.|
Immunocytochemical immunofluorescence analysis of methanol-fixed HT29 human cell line, labelling cannabinoid receptor II with ab45942 at 1/50 dilution incubated for 18 hours at 4°C in 1% BSA. Fixed cells were permeabilized with 0.25% Tween. Secondary used was a Goat anti-Rabbit polyclonal Alexa Fluor® 488. Counterstain is DAPI against nuclear DNA.
ab45942 staining Cannabinoid Receptor II in Rat ileum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Bouin's solution and blocked with 10% BSA for 10 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody for 17 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
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