Product nameCantharidin, Protein phosphatases 1 and 2A (PP1, PP2A) inhibitor
DescriptionProtein phosphatases 1 and 2A (PP1, PP2A) inhibitor
Biological descriptionProtein phosphatases 1 and 2A (PP1, PP2A) inhibitor (Ki values are 1.1 and 0.19 μM, respectively). Toxin found in Mylabris phalerata. Possesses various biological activities including anticancer, antitumor activity.
Storage instructionsStore at +4°C. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab120967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab7960 staining p21 in PANC-1 cells treated with cantharidin (ab120967), by ICC/IF. Increase of p21 expression correlates with increased concentration of cantharidin, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120967 (cantharidin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab7960 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab120967 has been referenced in 2 publications.
- Shomin-Levi H & Yarden O TheNeurospora crassaPP2A Regulatory Subunits RGB1 and B56 Are Required for Proper Growth and Development and Interact with the NDR Kinase COT1. Front Microbiol 8:1694 (2017). PubMed: 28928725
- Babiec WE et al. Basal levels of AMPA receptor GluA1 subunit phosphorylation at threonine 840 and serine 845 in hippocampal neurons. Learn Mem 23:127-33 (2016). PubMed: 26980779