Overview

  • Product name

  • Description

    Mouse polyclonal to CAP1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    CAP1 (NP_006358.1, 1 a.a. ~ 475 a.a) full-length human protein.

  • Positive control

    • Human colon membrane lysate A-431 membrane lysate Transfected 293T membrane lysate HeLa cells Methanol treated human HeLa cells Methanol treated human promonocytic THP-1 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab88446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
Flow Cyt Use at an assay dependent concentration.

ab37355 - Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity.
  • Sequence similarities

    Belongs to the CAP family.
    Contains 1 C-CAP/cofactor C-like domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adenylyl cyclase associated protein 1 antibody
    • Adenylyl cyclase-associated protein 1 antibody
    • CAP 1 antibody
    • CAP antibody
    • CAP, adenylate cyclase associated protein 1 (yeast) antibody
    • CAP1 antibody
    • CAP1 PEN antibody
    • CAP1-PEN antibody
    • CAP1_HUMAN antibody
    see all

Images

  • Anti-CAP1 antibody (ab88446) at 1 µg/ml + Human colon tissue at 50 µg

    Secondary
    Goat Anti-Mouse IgG at 1/2500 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa

  • Anti-CAP1 antibody (ab88446) at 1 µg/ml + A-431 cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG at 1/2500 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa

  • All lanes : Anti-CAP1 antibody (ab88446) at 1 µg/ml

    Lane 1 : Transfected 293T cell line
    Lane 2 : Non-transfected cell line

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG at 1/2500 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa

  • Immunofluorescence image staining human CAP1 in HeLa cells, using ab88446 at 10µg/ml.
  • FACS analysis of (a) negative control unstained, (b) negative control goat anti-mouse-FITC stained and (c) ab88446 stained methanol-treated human HeLa cells.
  • FACS analysis of (a) negative control unstained, (b) negative control goat anti-mouse-FITC stained and (c) ab88446 stained methanol-treated human promonocytic THP-1 cells.

References

This product has been referenced in:

  • Jin ZL  et al. CAP1-mediated actin cycling via ADF/cofilin proteins is essential for asymmetric division in mouse oocytes. J Cell Sci 131:N/A (2018). Read more (PubMed: 30404832) »
See 1 Publication for this product

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