Overview

  • Product name

  • Description

    Rabbit polyclonal to CAP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 266-462 of Human CAP1 (NP_001099000)

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji cell lines. Paraffin-embedded DLD1 xenograft. This antibody gave a positive result when used in the following formaldehyde/methanol fixed cell lines: PANC-1.

Properties

Applications

Our Abpromise guarantee covers the use of ab96354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IHC-P 1/100 - 1/500.
ICC/IF Use a concentration of 5 µg/ml.

Target

Images

  • All lanes : Anti-CAP1 antibody (ab96354) at 1/1000 dilution

    Lane 1 : Hep G2 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 52 kDa

  • ICC/IF image of ab96354 stained PANC-1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96354 at 5 ug/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab96354 at 1/500 dilution staining paraffin-embedded DLD1 xenograft

References

This product has been referenced in:

  • Rahmati M & Kazemi A Various exercise intensities differentially regulate GAP-43 and CAP-1 expression in the rat hippocampus. Gene 692:185-194 (2019). Read more (PubMed: 30682386) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - other (Brain)
Gel Running Conditions
Reduced Denaturing (7.5% gel)
Loading amount
200 µg
Specification
Brain
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 14 2017

Question
Answer

I am very pleased to hear you would like to accept our offer and test ab96354 in mouse. This code will give you $337 off your next order before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

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