Overview

  • Product name

    Anti-CAP1 antibody [EPR8338]
    See all CAP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8338] to CAP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CAP1 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • HepG2, 293T, K562 and Raji cell lysates; Human bladder carcinoma tissue; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity.
    • Sequence similarities

      Belongs to the CAP family.
      Contains 1 C-CAP/cofactor C-like domain.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Adenylyl cyclase associated protein 1 antibody
      • Adenylyl cyclase-associated protein 1 antibody
      • CAP 1 antibody
      • CAP antibody
      • CAP, adenylate cyclase associated protein 1 (yeast) antibody
      • CAP1 antibody
      • CAP1 PEN antibody
      • CAP1-PEN antibody
      • CAP1_HUMAN antibody
      see all

    Images

    • All lanes : Anti-CAP1 antibody [EPR8338] (ab133655) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 52 kDa
      Observed band size: 52 kDa

    • Immunocytochemistry/Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma) cells labelling CAP1 with purified ab133655 at a dilution of 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at dilution of 1/1000 was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue labelling CAP1 with ab133655 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue labelling CAP1 with ab133655 at 1/100 dilution.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Joshi P  et al. The membrane-associated fraction of cyclase associate protein 1 translocates to the cytosol upon platelet stimulation. Sci Rep 8:10804 (2018). Read more (PubMed: 30018317) »
    • Rüetschi U  et al. SILAC-Based Quantitative Proteomic Analysis of Diffuse Large B-Cell Lymphoma Patients. Int J Proteomics 2015:841769 (2015). WB . Read more (PubMed: 26060582) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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