Overview

  • Product name
    Anti-CAP1 antibody [EPR8339(B)]
    See all CAP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR8339(B)] to CAP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CAP1 aa 400 to the C-terminus.
    Database link: Q01518

  • Positive control
    • HeLa, 293T, A431, HepG2 and Caco-2 cell lysates; 293 cells; Prostate tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    Images

    • All lanes : Anti-CAP1 antibody [EPR8339(B)] (ab155079) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : Caco-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 52 kDa

    • Flow cytometric analysis of permeabilized 293 cells, labeling CAP1 with ab155079 at 1/10 dilution (red) or Rabiit IgG negative control (green).
    • Immunohistochemical analysis of paraffin-embedded prostate tissue, labeling CAP1 with ab155079 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab155079 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human gastric adenocarcinoma tissue using ab155079 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab155079 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue using ab155079 showing +ve staining.

    References

    This product has been referenced in:
    • Sato H  et al. Resistin upregulates chemokine production by fibroblast-like synoviocytes from patients with rheumatoid arthritis. Arthritis Res Ther 19:263 (2017). WB, IHC ; Human . Read more (PubMed: 29191223) »
    • Krochmal M  et al. Identification of novel molecular signatures of IgA nephropathy through an integrative -omics analysis. Sci Rep 7:9091 (2017). Read more (PubMed: 28831120) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HUES7 EScells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    HUES7 EScells
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C

    Abcam user community

    Verified customer

    Submitted Feb 04 2016

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEPG2)
    Gel Running Conditions
    Reduced Denaturing (12.5)
    Loading amount
    20 µg
    Specification
    HEPG2
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C

    Abcam user community

    Verified customer

    Submitted Nov 13 2015

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MET5A)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    100000 cells
    Specification
    MET5A
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C

    Abcam user community

    Verified customer

    Submitted Aug 19 2015

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse embryonic stem cell)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    100000 cells
    Specification
    mouse embryonic stem cell
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C

    Abcam user community

    Verified customer

    Submitted Dec 20 2013

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton X-100 in PBS

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Feb 21 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up