Product nameAnti-CAP1 antibody [EPR8339(B)]
See all CAP1 primary antibodies
DescriptionRabbit monoclonal [EPR8339(B)] to CAP1
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CAP1 aa 400 to the C-terminus.
Database link: Q01518
- HeLa, 293T, A431, HepG2 and Caco-2 cell lysates; 293 cells; Prostate tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab155079 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 52 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionDirectly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity.
Sequence similaritiesBelongs to the CAP family.
Contains 1 C-CAP/cofactor C-like domain.
Cellular localizationCell membrane.
- Information by UniProt
- Adenylyl cyclase associated protein 1 antibody
- Adenylyl cyclase-associated protein 1 antibody
- CAP 1 antibody
All lanes : Anti-CAP1 antibody [EPR8339(B)] (ab155079) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 52 kDa
Flow cytometric analysis of permeabilized 293 cells, labeling CAP1 with ab155079 at 1/10 dilution (red) or Rabiit IgG negative control (green).
Immunohistochemical analysis of paraffin-embedded prostate tissue, labeling CAP1 with ab155079 at 1/100 dilution.
Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab155079 showing +ve staining.
Immunohistochemical analysis of paraffin embedded Human gastric adenocarcinoma tissue using ab155079 showing +ve staining.
Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab155079 showing +ve staining.
Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue using ab155079 showing +ve staining.
This product has been referenced in:
- Sato H et al. Resistin upregulates chemokine production by fibroblast-like synoviocytes from patients with rheumatoid arthritis. Arthritis Res Ther 19:263 (2017). WB, IHC ; Human . Read more (PubMed: 29191223) »
- Krochmal M et al. Identification of novel molecular signatures of IgA nephropathy through an integrative -omics analysis. Sci Rep 7:9091 (2017). Read more (PubMed: 28831120) »