Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6378] to CAP2
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Product nameAnti-CAP2 antibody [EPR6378]
See all CAP2 primary antibodies
DescriptionRabbit monoclonal [EPR6378] to CAP2
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human CAP2 aa 450 to the C-terminus. The exact sequence is proprietary.
Database link: P40123
- Saos 2, A673, Human testis, Human heart, and Human fetal muscle lysates; Saos 2 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.80 x 10 -12 M Learn more about KD
Storage bufferpH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 53 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMay have a regulatory bifunctional role.
Sequence similaritiesBelongs to the CAP family.
Contains 1 C-CAP/cofactor C-like domain.
Cellular localizationCell membrane.
- Information by UniProt
- 2810452G09Rik antibody
- adenylate cyclase associated protein 2 antibody
- Adenylyl cyclase associated protein 2 antibody
All lanes : Anti-CAP2 antibody [EPR6378] (ab133558) at 1/1000 dilution
Lane 1 : Saos 2 cell lysate
Lane 2 : A673 cell lysate
Lane 3 : Human testis cell lysate
Lane 4 : Human heart cell lysate
Lane 5 : Human fetal muscle cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 53 kDa
Flow Cytometric analysis of permeabilized Saos 2 cells labelling CAP2 with ab133558 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133558 has not yet been referenced specifically in any publications.