Recombinant Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14519(B)] to CAPON/NOS1AP
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CAPON/NOS1AP antibody [EPR14519(B)]
See all CAPON/NOS1AP primary antibodies -
Description
Rabbit monoclonal [EPR14519(B)] to CAPON/NOS1AP -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain, Human cerebellum, Mouse brain and Rat brain lysates: MCF-7, HeLa, C6, Raw 264.7, PC-12 and NIH/3T3 cell lysates: SH-SY5Y and HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14519(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/130.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/50.
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WB |
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
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Notes |
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Flow Cyt (Intra)
1/130. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/50. |
WB
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
Target
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Function
Adapter protein involved in neuronal nitric-oxide (NO) synthesis regulation via its association with nNOS/NOS1. The complex formed with NOS1 and synapsins is necessary for specific NO and synapsin functions at a presynaptic level. Mediates an indirect interaction between NOS1 and RASD1 leading to enhance the ability of NOS1 to activate RASD1. Competes with DLG4 for interaction with NOS1, possibly affecting NOS1 activity by regulating the interaction between NOS1 and DLG4. -
Sequence similarities
Contains 1 PID domain. - Information by UniProt
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Database links
- Entrez Gene: 9722 Human
- Entrez Gene: 70729 Mouse
- Entrez Gene: 192363 Rat
- Omim: 605551 Human
- SwissProt: O75052 Human
- SwissProt: Q9D3A8 Mouse
- SwissProt: O54960 Rat
- Unigene: 655000 Human
see all -
Alternative names
- 6330408P19Rik antibody
- C terminal PDZ domain ligand of neuronal nitric oxide synthase (CAPON) antibody
- C terminal PDZ domain ligand of neuronal nitric oxide synthase antibody
see all
Images
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All lanes : Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686) at 1/1000 dilution
Lane 1 : Human fetal brain lysates
Lane 2 : Human cerebellum brain lysates
Lane 3 : MCF-7 cell lysates
Lane 4 : HeLa cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa -
Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686) at 1/10000 dilution + Human fetal brain lysates at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa -
All lanes : Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686) at 1/1000 dilution
Lane 1 : Mouse brain lysates
Lane 2 : Rat brain lysates
Lane 3 : C6 cell lysates
Lane 4 : Raw 264.7 cell lysates
Lane 5 : PC-12 cell lysates
Lane 6 : NIH/3T3 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa -
Immunofluorescence analysis of 4% paraformaldehyde-fixed SH-SY5Y cells labeling CAPON/NOS1AP with ab190686 at 1/50 dilution. Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody (red). Slide is counter stained with Dapi (blue).
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Intracellular flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling CAPON/NOS1AP with ab190686 at 1/130 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab190686 has been referenced in 3 publications.
- Shi HJ et al. Requirement of hippocampal DG nNOS-CAPON dissociation for the anxiolytic and antidepressant effects of fluoxetine. Theranostics 12:3656-3675 (2022). PubMed: 35664081
- Zhu LJ et al. nNOS-CAPON blockers produce anxiolytic effects by promoting synaptogenesis in chronic stress-induced animal models of anxiety. Br J Pharmacol 177:3674-3690 (2020). PubMed: 32343840
- Zhou Z et al. Phosphorylated neuronal nitric oxide synthase in neuropathic pain in rats. Int J Clin Exp Pathol 8:12748-56 (2015). PubMed: 26722464