Recombinant
RabMAb

Recombinant Anti-CAPON/NOS1AP antibody [EPR14519(B)] - BSA and Azide free (ab236134)

Overview

  • Product name

    Anti-CAPON/NOS1AP antibody [EPR14519(B)] - BSA and Azide free
    See all CAPON/NOS1AP primary antibodies
  • Description

    Rabbit monoclonal [EPR14519(B)] to CAPON/NOS1AP - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CAPON/NOS1AP aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: O75052

  • Positive control

    • ICC/IF: SH-SY5Y cells.
  • General notes

    ab236134 is the carrier-free version of ab190686 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab236134 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as CAPON

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB Use at an assay dependent concentration. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).

Target

  • Function

    Adapter protein involved in neuronal nitric-oxide (NO) synthesis regulation via its association with nNOS/NOS1. The complex formed with NOS1 and synapsins is necessary for specific NO and synapsin functions at a presynaptic level. Mediates an indirect interaction between NOS1 and RASD1 leading to enhance the ability of NOS1 to activate RASD1. Competes with DLG4 for interaction with NOS1, possibly affecting NOS1 activity by regulating the interaction between NOS1 and DLG4.
  • Sequence similarities

    Contains 1 PID domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • 6330408P19Rik antibody
    • C terminal PDZ domain ligand of neuronal nitric oxide synthase (CAPON) antibody
    • C terminal PDZ domain ligand of neuronal nitric oxide synthase antibody
    • C terminal PDZ ligand of neuronal nitric oxide synthase protein antibody
    • C-terminal PDZ ligand of neuronal nitric oxide synthase protein antibody
    • CAPON antibody
    • CAPON_HUMAN antibody
    • Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein antibody
    • Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein antibody
    • Ligand of neuronal nitric oxide synthase with carboxyl terminal PDZ domain antibody
    • MGC138500 antibody
    • Nitric oxide synthase 1 (neuronal) adaptor protein antibody
    • Nitric oxide synthase 1 adaptor protein antibody
    • Nos1ap antibody
    see all

Images

  • Flow Cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling CAPON/NOS1AP with ab190686 at 1/130 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190686).

  • Immunofluorescence analysis of 4% paraformaldehyde-fixed SH-SY5Y cells labeling CAPON/NOS1AP with ab190686 at 1/50 dilution. Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody (red). Slide is counter stained with Dapi (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190686).

References

ab236134 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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