Anti-Caprin-1 antibody (ab205377)
Key features and details
- Goat polyclonal to Caprin-1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Caprin-1 antibody
See all Caprin-1 primary antibodies -
Description
Goat polyclonal to Caprin-1 -
Host species
Goat -
Specificity
ab205377 is expected to recognize both reported isoforms (NP_005889.3; NP_976240.1). -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig -
Immunogen
Synthetic peptide corresponding to Human Caprin-1 aa 450-550 (internal sequence) (Cysteine residue).
Database link: Q14444 -
Positive control
- HeLa and jurkat cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab205377 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab205377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 80,140 kDa (predicted molecular weight: 78 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 80,140 kDa (predicted molecular weight: 78 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
May regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the caprin family. -
Cellular localization
Cytoplasm > cytosol. Cell projection > dendrite. Associated with RNA granules. - Information by UniProt
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Database links
- Entrez Gene: 535571 Cow
- Entrez Gene: 607420 Dog
- Entrez Gene: 4076 Human
- Entrez Gene: 53872 Mouse
- Entrez Gene: 100512646 Pig
- Entrez Gene: 362173 Rat
- Omim: 601178 Human
- SwissProt: Q1LZB6 Cow
see all -
Alternative names
- activation/proliferation-associated protein 1 antibody
- CAPR1_HUMAN antibody
- Caprin-1 antibody
see all
Images
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All lanes : Anti-Caprin-1 antibody (ab205377) at 0.3 µg/ml
Lane 1 : Jurkat cell lysate (in RIPA buffer)
Lane 2 : HeLa cell lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 78 kDa
Observed band size: 140,80 kDa why is the actual band size different from the predicted?The ~140 kDa band corresponds to the reported p137 protein (Ellis and Luzio, J Biol Chem. 1995 Sep 1;270(35):20717-23.PMID: 7657653).
Datasheets and documents
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Datasheet download
References (0)
ab205377 has not yet been referenced specifically in any publications.