Key features and details
- Goat polyclonal to Caprin-1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Caprin-1 antibody
See all Caprin-1 primary antibodies
DescriptionGoat polyclonal to Caprin-1
Specificityab205377 is expected to recognize both reported isoforms (NP_005889.3; NP_976240.1).
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig
- HeLa and jurkat cell lysates.
Previously labelled as GPIP137.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab205377 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab205377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 80,140 kDa (predicted molecular weight: 78 kDa).
1 hour primary incubation is recommended for this product.
FunctionMay regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types.
Sequence similaritiesBelongs to the caprin family.
Cellular localizationCytoplasm > cytosol. Cell projection > dendrite. Associated with RNA granules.
- Information by UniProt
- activation/proliferation-associated protein 1 antibody
- CAPR1_HUMAN antibody
- Caprin-1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human cortex staining Caprin-1 with ab205377 at 5 μg/ml
All lanes : Anti-Caprin-1 antibody (ab205377) at 0.3 µg/ml
Lane 1 : Jurkat cell lysate (in RIPA buffer)
Lane 2 : HeLa cell lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 78 kDa
Observed band size: 140,80 kDa why is the actual band size different from the predicted?
The ~140 kDa band corresponds to the reported p137 protein (Ellis and Luzio, J Biol Chem. 1995 Sep 1;270(35):20717-23.PMID: 7657653).
ab205377 has not yet been referenced specifically in any publications.