Overview

  • Product name

  • Description

    Goat polyclonal to Caprin-1
  • Host species

    Goat
  • Specificity

    ab205377 is expected to recognize both reported isoforms (NP_005889.3; NP_976240.1).
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Caprin-1 aa 456-466 (internal sequence) (Cysteine residue).
    Sequence:

    EQRPQKEPIDQ


    Database link: Q14444

  • Positive control

    • HeLa and jurkat cell lysates.
  • General notes

    Previously labelled as GPIP137.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab205377 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab205377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 80,140 kDa (predicted molecular weight: 78 kDa).

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human cortex staining Caprin-1 with ab205377 at 5 μg/ml

  • All lanes : Anti-Caprin-1 antibody (ab205377) at 0.3 µg/ml

    Lane 1 : Jurkat cell lysate (in RIPA buffer)
    Lane 2 : HeLa cell lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 78 kDa
    Observed band size: 140,80 kDa
    why is the actual band size different from the predicted?



    The ~140 kDa band corresponds to the reported p137 protein (Ellis and Luzio, J Biol Chem. 1995 Sep 1;270(35):20717-23.PMID: 7657653).

References

ab205377 has not yet been referenced specifically in any publications.

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