Key features and details
- Rabbit polyclonal to Caprin-1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Caprin-1 antibody
See all Caprin-1 primary antibodies
DescriptionRabbit polyclonal to Caprin-1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Synthetic peptide corresponding to Human Caprin-1 aa 550-600. NP_005889.3
Database link: Q14444
- WB: HeLa, HEK-293T, Jurkat and NIH/3T3 whole cell lysate. IP: Caprin-1 IP in HeLa whole cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to Caprin-1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 78 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMay regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types.
Sequence similaritiesBelongs to the caprin family.
Cellular localizationCytoplasm > cytosol. Cell projection > dendrite. Associated with RNA granules.
- Information by UniProt
- activation/proliferation-associated protein 1 antibody
- CAPR1_HUMAN antibody
- Caprin-1 antibody
All lanes : Anti-Caprin-1 antibody (ab241071) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 78 kDa
Exposure time: 30 seconds
Caprin-1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
Lane 1: ab241071 used for IP at 6 µg/mg lysate. For WB 1 µg/ml.
Lane 2: Control IgG IP.
Chemiluminescence detection: 3 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241071 has not yet been referenced specifically in any publications.