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    caprin-1-antibody-ab241071.pdf

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Cell Biology Cell Cycle Cell Division Other cell division antibodies
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Anti-Caprin-1 antibody (ab241071)

  • Datasheet
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Abpromise

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Western blot - Anti-Caprin-1 antibody (ab241071)
  • Immunoprecipitation - Anti-Caprin-1 antibody (ab241071)

Key features and details

  • Rabbit polyclonal to Caprin-1
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-Caprin-1 antibody
    See all Caprin-1 primary antibodies
  • Description

    Rabbit polyclonal to Caprin-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to Human Caprin-1 aa 550-600. NP_005889.3
    Database link: Q14444

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat and NIH/3T3 whole cell lysate. IP: Caprin-1 IP in HeLa whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to Caprin-1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Other cell division antibodies

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • NIH 3T3 whole cell lysate (ab7179)
    • Jurkat whole cell lysate (ab7899)

Applications

Our Abpromise guarantee covers the use of ab241071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 78 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    May regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the caprin family.
  • Cellular localization

    Cytoplasm > cytosol. Cell projection > dendrite. Associated with RNA granules.
  • Target information above from: UniProt accession Q14444 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 535571 Cow
    • Entrez Gene: 4076 Human
    • Entrez Gene: 53872 Mouse
    • Entrez Gene: 362173 Rat
    • Omim: 601178 Human
    • SwissProt: Q1LZB6 Cow
    • SwissProt: Q14444 Human
    • SwissProt: Q60865 Mouse
    • SwissProt: Q5M9G3 Rat
    • Unigene: 471818 Human
    • Unigene: 427589 Mouse
    • Unigene: 477287 Mouse
    • Unigene: 22572 Rat
    see all
  • Alternative names

    • activation/proliferation-associated protein 1 antibody
    • CAPR1_HUMAN antibody
    • Caprin-1 antibody
    • CAPRIN1 antibody
    • Cell cycle-associated protein 1 antibody
    • Cytoplasmic activation- and proliferation-associated protein 1 antibody
    • cytoplasmic activation/proliferation-associated protein-1 antibody
    • GPI-anchored membrane protein 1 antibody
    • GPI-anchored protein p137 antibody
    • GPI-p137 antibody
    • GPIAP1 antibody
    • GPIP137 antibody
    • M11S1 antibody
    • Membrane component chromosome 11 surface marker 1 antibody
    • p137GPI antibody
    • RNA granule protein 105 antibody
    • RNG105 antibody
    see all

Images

  • Western blot - Anti-Caprin-1 antibody (ab241071)
    Western blot - Anti-Caprin-1 antibody (ab241071)
    All lanes : Anti-Caprin-1 antibody (ab241071) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 78 kDa


    Exposure time: 30 seconds
  • Immunoprecipitation - Anti-Caprin-1 antibody (ab241071)
    Immunoprecipitation - Anti-Caprin-1 antibody (ab241071)

    Caprin-1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

    Lane 1: ab241071 used for IP at 6 µg/mg lysate. For WB 1 µg/ml.
    Lane 2: Control IgG IP.

    Chemiluminescence detection: 3 seconds.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab241071? Please let us know so that we can cite the reference in this datasheet.

    ab241071 has not yet been referenced specifically in any publications.

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