Anti-Caprin-1 antibody (ab241071)
Key features and details
- Rabbit polyclonal to Caprin-1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Caprin-1 antibody
See all Caprin-1 primary antibodies -
Description
Rabbit polyclonal to Caprin-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
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Immunogen
Synthetic peptide corresponding to Human Caprin-1 aa 550-600. NP_005889.3
Database link: Q14444 -
Positive control
- WB: HeLa, HEK-293T, Jurkat and NIH/3T3 whole cell lysate. IP: Caprin-1 IP in HeLa whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to Caprin-1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab241071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 78 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
May regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the caprin family. -
Cellular localization
Cytoplasm > cytosol. Cell projection > dendrite. Associated with RNA granules. - Information by UniProt
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Database links
- Entrez Gene: 535571 Cow
- Entrez Gene: 4076 Human
- Entrez Gene: 53872 Mouse
- Entrez Gene: 362173 Rat
- Omim: 601178 Human
- SwissProt: Q1LZB6 Cow
- SwissProt: Q14444 Human
- SwissProt: Q60865 Mouse
see all -
Alternative names
- activation/proliferation-associated protein 1 antibody
- CAPR1_HUMAN antibody
- Caprin-1 antibody
see all
Images
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Western blot - Anti-Caprin-1 antibody (ab241071)All lanes : Anti-Caprin-1 antibody (ab241071) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 78 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-Caprin-1 antibody (ab241071)Caprin-1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
Lane 1: ab241071 used for IP at 6 µg/mg lysate. For WB 1 µg/ml.
Lane 2: Control IgG IP.Chemiluminescence detection: 3 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab241071 has not yet been referenced specifically in any publications.
