Anti-Caprin-1 antibody (ab241071)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Caprin-1 antibody
See all Caprin-1 primary antibodies -
Description
Rabbit polyclonal to Caprin-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
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Immunogen
Synthetic peptide corresponding to Human Caprin-1 aa 550-600. NP_005889.3
Database link: Q14444 -
Positive control
- WB: HeLa, HEK-293T, Jurkat and NIH/3T3 whole cell lysate. IP: Caprin-1 IP in HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to Caprin-1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab241071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 78 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
May regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the caprin family. -
Cellular localization
Cytoplasm > cytosol. Cell projection > dendrite. Associated with RNA granules. - Information by UniProt
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Database links
- Entrez Gene: 535571 Cow
- Entrez Gene: 4076 Human
- Entrez Gene: 53872 Mouse
- Entrez Gene: 362173 Rat
- Omim: 601178 Human
- SwissProt: Q1LZB6 Cow
- SwissProt: Q14444 Human
- SwissProt: Q60865 Mouse
see all -
Alternative names
- activation/proliferation-associated protein 1 antibody
- CAPR1_HUMAN antibody
- Caprin-1 antibody
see all
Images
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Western blot - Anti-Caprin-1 antibody (ab241071)All lanes : Anti-Caprin-1 antibody (ab241071) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 78 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-Caprin-1 antibody (ab241071)Caprin-1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
Lane 1: ab241071 used for IP at 6 µg/mg lysate. For WB 1 µg/ml.
Lane 2: Control IgG IP.Chemiluminescence detection: 3 seconds.
Datasheets and documents
References
ab241071 has not yet been referenced specifically in any publications.
