Overview

  • Product name

  • Description

    Rabbit polyclonal to Caprin-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Caprin-1 aa 450-500. The exact sequence is proprietary. NP_005889.3
    Database link: Q14444

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat and NIH/3T3 whole cell lysate. IP: Caprin-1 IP in HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245582 was affinity purified using an epitope specific to Caprin-1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    May regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the caprin family.
  • Cellular localization

    Cytoplasm > cytosol. Cell projection > dendrite. Associated with RNA granules.
  • Information by UniProt
  • Database links

  • Alternative names

    • activation/proliferation-associated protein 1 antibody
    • CAPR1_HUMAN antibody
    • Caprin-1 antibody
    • CAPRIN1 antibody
    • Cell cycle-associated protein 1 antibody
    • Cytoplasmic activation- and proliferation-associated protein 1 antibody
    • cytoplasmic activation/proliferation-associated protein-1 antibody
    • GPI-anchored membrane protein 1 antibody
    • GPI-anchored protein p137 antibody
    • GPI-p137 antibody
    • GPIAP1 antibody
    • GPIP137 antibody
    • M11S1 antibody
    • Membrane component chromosome 11 surface marker 1 antibody
    • p137GPI antibody
    • RNA granule protein 105 antibody
    • RNG105 antibody
    see all

Images

  • All lanes : Anti-Caprin-1 antibody (ab245582) at 0.1 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Exposure time: 3 seconds
  • Caprin-1 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

    ab245582 used for IP at 6 µg/mg lysate. For WB 1 µg/ml.

    Lane 1: ab245582 IP in HeLa whole cell lysate.
    Lane 2: Control IgG in HeLa whole cell lysate.
    Chemiluminescence detection: 1 second.

References

ab245582 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab245582.
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