Key features and details
- Rabbit polyclonal to Caprin-1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Caprin-1 antibody
See all Caprin-1 primary antibodies
DescriptionRabbit polyclonal to Caprin-1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human Caprin-1 aa 450-500. The exact sequence is proprietary. NP_005889.3
Database link: Q14444
- WB: HeLa, HEK-293T, Jurkat and NIH/3T3 whole cell lysate. IP: Caprin-1 IP in HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245582 was affinity purified using an epitope specific to Caprin-1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMay regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types.
Sequence similaritiesBelongs to the caprin family.
Cellular localizationCytoplasm > cytosol. Cell projection > dendrite. Associated with RNA granules.
- Information by UniProt
- activation/proliferation-associated protein 1 antibody
- CAPR1_HUMAN antibody
- Caprin-1 antibody
All lanes : Anti-Caprin-1 antibody (ab245582) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Exposure time: 3 seconds
Caprin-1 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab245582 used for IP at 6 µg/mg lysate. For WB 1 µg/ml.
Lane 1: ab245582 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Chemiluminescence detection: 1 second.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245582 has not yet been referenced specifically in any publications.