Overview

  • Product name
  • Description
    Rabbit polyclonal to Caprin-1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Caprin-1 aa 615-649 (C terminal) conjugated to keyhole limpet haemocyanin.

  • Positive control
    • Human breast carcinoma
  • General notes

    Previously labelled as GPIP137.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
IHC-P 1/50 - 1/100.

Target

  • Function
    May regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the caprin family.
  • Cellular localization
    Cytoplasm > cytosol. Cell projection > dendrite. Associated with RNA granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • activation/proliferation-associated protein 1 antibody
    • CAPR1_HUMAN antibody
    • Caprin-1 antibody
    • CAPRIN1 antibody
    • Cell cycle-associated protein 1 antibody
    • Cytoplasmic activation- and proliferation-associated protein 1 antibody
    • cytoplasmic activation/proliferation-associated protein-1 antibody
    • GPI-anchored membrane protein 1 antibody
    • GPI-anchored protein p137 antibody
    • GPI-p137 antibody
    • GPIAP1 antibody
    • GPIP137 antibody
    • M11S1 antibody
    • Membrane component chromosome 11 surface marker 1 antibody
    • p137GPI antibody
    • RNA granule protein 105 antibody
    • RNG105 antibody
    see all

Images

  • Anti-Caprin-1 antibody (ab38859) + HL-60 cell lysate

    Observed band size: 72 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 24 kDa. We are unsure as to the identity of these extra bands.



    ab 38859 is used in Western blot to detect Caprin-1 in HL-60 cell lysate (dilution 1:100-1:500)

  • Ab38859 at 1/50 dilution staining human breast carcinoma for Caprin-1 expression. (A peroxidase-conjugated secondary antibody, followed by AEC staining was used).

References

ab38859 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

I am very pleased to hear you would like to accept our offer and test ab38859 in murine cell lines in WB. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for WB with mouse samples and include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
CLIP
Sample
Human Cell lysate - whole cell (HEK 293T)
Specification
HEK 293T
Type
iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control
hnRNP C (sc-15386) bound protein A dynabeads, 0.1 mg/ml (antibody/beads)
Negative control
Protein A dynabeads without antibody bound
Immuno-precipitation step
Other - Protein A Dynabeads

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Verified customer

Submitted Jun 02 2011

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