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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Binding Proteins
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RecombinantRabMAb

Recombinant Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)

  • Datasheet
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Western blot - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)
  • Immunoprecipitation - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)
  • Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11210-4R] to CAPZA1
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-CAPZA1 antibody [EPR11210-4R]
    See all CAPZA1 primary antibodies
  • Description

    Rabbit monoclonal [EPR11210-4R] to CAPZA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human CAPZA1. The exact sequence is proprietary.
    Database link: P52907

  • Positive control

    • HeLa, Jurkat, 293T and HepG2 cell lysates; Human muscle and Human urinary bladder carcinoma tissues.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11210-4R
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins

Associated products

  • Alternative Versions

    • Anti-CAPZA1 antibody [EPR11210-4R] - BSA and Azide free (ab249883)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HepG2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)

Applications

Our Abpromise guarantee covers the use of ab175233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.
    • Sequence similarities

      Belongs to the F-actin-capping protein alpha subunit family.
    • Cellular localization

      Cytoplasm > cytoskeleton.
    • Target information above from: UniProt accession P52907 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 829 Human
      • Entrez Gene: 12340 Mouse
      • Entrez Gene: 691149 Rat
      • Omim: 601580 Human
      • SwissProt: P52907 Human
      • SwissProt: P47753 Mouse
      • SwissProt: B2GUZ5 Rat
      • Unigene: 727604 Human
      • Unigene: 19142 Mouse
      • Unigene: 13838 Rat
      see all
    • Alternative names

      • Cap Z antibody
      • Cappa 1 antibody
      • Cappa1 antibody
      • Capping protein (actin filament) muscle Z line alpha 1 antibody
      • Capping protein alpha 1 antibody
      • Capping protein muscle Z line alpha 1 antibody
      • CapZ alpha 1 antibody
      • CapZ alpha-1 antibody
      • CAPZ antibody
      • CAPZA 1 antibody
      • Capza1 antibody
      • CAZ 1 antibody
      • CAZ1 antibody
      • CAZA1_HUMAN antibody
      • F actin capping protein alpha 1 subunit antibody
      • F-actin-capping protein subunit alpha-1 antibody
      see all

    Images

    • Western blot - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)
      Western blot - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)
      All lanes : Anti-CAPZA1 antibody [EPR11210-4R] (ab175233) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 33 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)

      Immunohistochemical analysis of paraffin embedded Human muscle tissue labeling CAPZA1 with ab175233 at 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)
      Immunoprecipitation - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)

      Immunoprecipitation of CAPZA1 from HepG2 cell lysate using ab175233 at 1/10.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)

      Immunohistochemical analysis of paraffin embedded Human urinary bladder carcinoma tissue labeling CAPZA1 with ab175233 at 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)
      Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab175233? Please let us know so that we can cite the reference in this datasheet.

    ab175233 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab175233.
    Please use the links above to contact us or submit feedback about this product.

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