Recombinant
RabMAb

Recombinant Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)

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Overview

  • Product name

    Anti-CAPZA1 antibody [EPR11210-4R]
    See all CAPZA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11210-4R] to CAPZA1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human CAPZA1. The exact sequence is proprietary.
    Database link: P52907

  • Positive control

    • HeLa, Jurkat, 293T and HepG2 cell lysates; Human muscle and Human urinary bladder carcinoma tissues.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11210-4R

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.

    • Target

    • Function

      F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.

    • Sequence similarities

      Belongs to the F-actin-capping protein alpha subunit family.

    • Cellular localization

      Cytoplasm > cytoskeleton.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Cap Z antibody
      • Cappa 1 antibody
      • Cappa1 antibody
      • Capping protein (actin filament) muscle Z line alpha 1 antibody
      • Capping protein alpha 1 antibody
      • Capping protein muscle Z line alpha 1 antibody
      • CapZ alpha 1 antibody
      • CapZ alpha-1 antibody
      • CAPZ antibody
      • CAPZA 1 antibody
      • Capza1 antibody
      • CAZ 1 antibody
      • CAZ1 antibody
      • CAZA1_HUMAN antibody
      • F actin capping protein alpha 1 subunit antibody
      • F-actin-capping protein subunit alpha-1 antibody
      see all

    Images

    • Western blot - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)
      Western blot - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)
      All lanes : Anti-CAPZA1 antibody [EPR11210-4R] (ab175233) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 33 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)

      Immunohistochemical analysis of paraffin embedded Human muscle tissue labeling CAPZA1 with ab175233 at 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)
      Immunoprecipitation - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)

      Immunoprecipitation of CAPZA1 from HepG2 cell lysate using ab175233 at 1/10.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)

      Immunohistochemical analysis of paraffin embedded Human urinary bladder carcinoma tissue labeling CAPZA1 with ab175233 at 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab175233 has not yet been referenced specifically in any publications.

    Publishing research using ab175233? Please let us know so that we can cite the reference in this datasheet.

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