Name: Anti-CAPZA1 antibody [EPR11210]
Brand: RabMAb
SKU: ab166892

Product name

Anti-CAPZA1 antibody [EPR11210]
See all CAPZA1 primary antibodies
Description

Rabbit monoclonal [EPR11210] to CAPZA1
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, IP, Flow Cytmore details
Unsuitable for: ICC
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment corresponding to residues in Human CAPZA1 (UniProt ID: P52907).
Positive control
- HeLa, Jurkat, 293T and HepG2 whole cell lysate (ab7900); Human cervical carcinoma and Human muscle tissues; Jurkat cells.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11210
Isotype

IgG
Research areas

Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
Related Products
- Recombinant Human CAPZA1 protein (ab132127)

Our Abpromise guarantee covers the use of ab166892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
IHC-P		1/100 - 1/250.
IP		1/10 - 1/100.
Flow Cyt		1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes

Is unsuitable for ICC.

Function

F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.
Sequence similarities

Belongs to the F-actin-capping protein alpha subunit family.
Cellular localization

Cytoplasm > cytoskeleton.
Target information above from: UniProt accession P52907 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 829 Human
- Entrez Gene: 12340 Mouse
- Entrez Gene: 691149 Rat
- Omim: 601580 Human
- SwissProt: P52907 Human
- SwissProt: P47753 Mouse
- SwissProt: B2GUZ5 Rat
- Unigene: 727604 Human
- Unigene: 19142 Mouse
- Unigene: 13838 Rat
see all
Alternative names
- Cap Z antibody
- Cappa 1 antibody
- Cappa1 antibody
- Capping protein (actin filament) muscle Z line alpha 1 antibody
- Capping protein alpha 1 antibody
- Capping protein muscle Z line alpha 1 antibody
- CapZ alpha 1 antibody
- CapZ alpha-1 antibody
- CAPZ antibody
- CAPZA 1 antibody
- Capza1 antibody
- CAZ 1 antibody
- CAZ1 antibody
- CAZA1_HUMAN antibody
- F actin capping protein alpha 1 subunit antibody
- F-actin-capping protein subunit alpha-1 antibody
see all

Western blot - Anti-CAPZA1 antibody [EPR11210] (ab166892)

All lanes : Anti-CAPZA1 antibody [EPR11210] (ab166892) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 33 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)

ab166892 showing +ve staining in Human thyroid gland carcinoma.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)

ab166892 showing +ve staining in Human urinary bladder carcinoma.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)

ab166892 showing +ve staining in Human colonic adenocarcinoma.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)

ab166892 showing +ve staining in Human normal brain.
Immunoprecipitation - Anti-CAPZA1 antibody [EPR11210] (ab166892)

ab166892, at 1/1000 dilution, staining CAPZA1 in HepG2 cell lysate immunoprecipitated using ab166892 at 1/10.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)

Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labeling CAPZA1 with ab166892 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)

Immunohistochemical analysis of paraffin embedded Human muscle tissue labeling CAPZA1 with ab166892 at 1/100 dilution.
Flow Cytometry - Anti-CAPZA1 antibody [EPR11210] (ab166892)

Flow cytometric analysis of permeabilized Jurkat cells using ab166892 at a dilution of 1/10 (red) compared to a rabbit IgG (negative) (green).

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Western blot protocols

Datasheet

This product has been referenced in:

Su X et al. Embryonic lethality in mice lacking Trim59 due to impaired gastrulation development. Cell Death Dis 9:302 (2018). Read more (PubMed: 29467473) »

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Anti-CAPZA1 antibody [EPR11210] (ab166892)

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