Anti-CAPZA2 antibody (ab101451)
Key features and details
- Rabbit polyclonal to CAPZA2
- Suitable for: WB
- Reacts with: Mouse, Human, Zebrafish
- Isotype: IgG
Overview
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Product name
Anti-CAPZA2 antibody
See all CAPZA2 primary antibodies -
Description
Rabbit polyclonal to CAPZA2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human, Zebrafish
Predicted to work with: Rat, Cow, Dog, Monkey
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 134-268 of Human CAPZA2 (AAH05338).
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Positive control
- A549, HeLa, HepG2, HCT116, Adult zebrafish and NIH-3T3 whole cell lysate
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab101451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 33 kDa. |
Target
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Function
F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. -
Sequence similarities
Belongs to the F-actin-capping protein alpha subunit family. - Information by UniProt
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Database links
- Entrez Gene: 493728 Cow
- Entrez Gene: 503863 Dog
- Entrez Gene: 830 Human
- Entrez Gene: 12343 Mouse
- Entrez Gene: 493810 Rat
- Omim: 601571 Human
- SwissProt: Q5E997 Cow
- SwissProt: A0M8V0 Dog
see all -
Alternative names
- CAPPA2 antibody
- Capping protein (actin filament) muscle Z line, alpha 2 antibody
- CapZ alpha 2 antibody
see all
Images
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Western blot - Anti-CAPZA2 antibody (ab101451)All lanes : Anti-CAPZA2 antibody (ab101451) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : HCT116 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 33 kDa
12% SDS PAGE -
Western blot - Anti-CAPZA2 antibody (ab101451)Anti-CAPZA2 antibody (ab101451) at 1/1000 dilution + Adult zebrafish whole cell lysate at 10 µg
Predicted band size: 33 kDa
12% SDS PAGE -
Western blot - Anti-CAPZA2 antibody (ab101451)Anti-CAPZA2 antibody (ab101451) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg
Predicted band size: 33 kDa
12% SDS PAGE
References (0)
ab101451 has not yet been referenced specifically in any publications.
