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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Binding Proteins
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Anti-CAPZA2 antibody (ab101451)

  • Datasheet
  • SDS
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Western blot - Anti-CAPZA2 antibody (ab101451)
  • Western blot - Anti-CAPZA2 antibody (ab101451)
  • Western blot - Anti-CAPZA2 antibody (ab101451)

Key features and details

  • Rabbit polyclonal to CAPZA2
  • Suitable for: WB
  • Reacts with: Mouse, Human, Zebrafish
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-CAPZA2 antibody
    See all CAPZA2 primary antibodies
  • Description

    Rabbit polyclonal to CAPZA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human, Zebrafish
    Predicted to work with: Rat, Cow, Dog, Monkey
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 134-268 of Human CAPZA2 (AAH05338).

  • Positive control

    • A549, HeLa, HepG2, HCT116, Adult zebrafish and NIH-3T3 whole cell lysate
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • NIH 3T3 whole cell lysate (ab7179)

Applications

Our Abpromise guarantee covers the use of ab101451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 33 kDa.

Target

  • Function

    F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.
  • Sequence similarities

    Belongs to the F-actin-capping protein alpha subunit family.
  • Target information above from: UniProt accession P47755 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 493728 Cow
    • Entrez Gene: 503863 Dog
    • Entrez Gene: 830 Human
    • Entrez Gene: 12343 Mouse
    • Entrez Gene: 493810 Rat
    • Omim: 601571 Human
    • SwissProt: Q5E997 Cow
    • SwissProt: A0M8V0 Dog
    • SwissProt: P47755 Human
    • SwissProt: P47754 Mouse
    • SwissProt: Q3T1K5 Rat
    • Unigene: 446123 Human
    • Unigene: 392504 Mouse
    • Unigene: 17868 Rat
    see all
  • Alternative names

    • CAPPA2 antibody
    • Capping protein (actin filament) muscle Z line, alpha 2 antibody
    • CapZ alpha 2 antibody
    • CapZ alpha-2 antibody
    • CAPZ antibody
    • CAPZA2 antibody
    • CAZA2_HUMAN antibody
    • F actin capping protein alpha 2 subunit antibody
    • F actin capping protein subunit alpha 2 antibody
    • F-actin-capping protein subunit alpha-2 antibody
    see all

Images

  • Western blot - Anti-CAPZA2 antibody (ab101451)
    Western blot - Anti-CAPZA2 antibody (ab101451)
    All lanes : Anti-CAPZA2 antibody (ab101451) at 1/1000 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : HCT116 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 33 kDa



    12% SDS PAGE
  • Western blot - Anti-CAPZA2 antibody (ab101451)
    Western blot - Anti-CAPZA2 antibody (ab101451)
    Anti-CAPZA2 antibody (ab101451) at 1/1000 dilution + Adult zebrafish whole cell lysate at 10 µg

    Predicted band size: 33 kDa



    12% SDS PAGE
  • Western blot - Anti-CAPZA2 antibody (ab101451)
    Western blot - Anti-CAPZA2 antibody (ab101451)
    Anti-CAPZA2 antibody (ab101451) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg

    Predicted band size: 33 kDa



    12% SDS PAGE

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab101451? Please let us know so that we can cite the reference in this datasheet.

    ab101451 has not yet been referenced specifically in any publications.

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