Key features and details
- Rabbit polyclonal to CAPZA2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-CAPZA2 antibody
See all CAPZA2 primary antibodies
DescriptionRabbit polyclonal to CAPZA2
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human CAPZA2 aa 1-286.
MADLEEQLSDEEKVRIAAKFIIHAPPGEFNEVFNDVRLLLNNDNLLREGA AHAFAQYNLDQFTPVKIEGYEDQVLITEHGDLGNGKFLDPKNRICFKFDH LRKEATDPRPCEVENAVESWRTSVETALRAYVKEHYPNGVCTVYGKKIDG QQTIIACIESHQFQAKNFWNGRWRSEWKFTITPSTTQVVGILKIQVHYYE DGNVQLVSHKDIQDSLTVSNEVQTAKEFIKIVEAAENEYQTAISENYQTM SDTTFKALRRQLPVTRTKIDWNKILSYKIGKEMQNA
Database link: P47755
- HeLa, THP1, 22RV1, Mouse brain and Mouse heart lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab175378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 33 kDa.|
FunctionF-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.
Sequence similaritiesBelongs to the F-actin-capping protein alpha subunit family.
- Information by UniProt
- CAPPA2 antibody
- Capping protein (actin filament) muscle Z line, alpha 2 antibody
- CapZ alpha 2 antibody
All lanes : Anti-CAPZA2 antibody (ab175378) at 1/500 dilution
Lane 1 : HeLa lysate
Lane 2 : THP1 lysate
Lane 3 : 22RV1 lysate
Lane 4 : Mouse brain lysate
Lane 5 : Mouse heart lysate
Predicted band size: 33 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling CAPZA2 with ab175378 at 1/200. Magnification: 400x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling CAPZA2 with ab175378 at 1/200. Magnification: 400x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CAPZA2 with ab175378 at 1/200. Magnification: 400x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling CAPZA2 with ab175378 at 1/200. Magnification: 400x.
Immunocytochemistry/Immunofluorescence analysis of HepG2 cells using ab175378. Blue DAPI for nuclear staining.
ab175378 has been referenced in 1 publication.
- Peris-Frau P et al. Freezing-Thawing Procedures Remodel the Proteome of Ram Sperm before and after In Vitro Capacitation. Int J Mol Sci 20:N/A (2019). PubMed: 31533312