Key features and details
- Rabbit polyclonal to CAPZB
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CAPZB antibody
See all CAPZB primary antibodies
DescriptionRabbit polyclonal to CAPZB
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Pig, Orangutan
- RT4 and U251 MG cell line lysates; Human liver and tonsil tissue lysates; U251 MG cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab220669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 31 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionF-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.
Sequence similaritiesBelongs to the F-actin-capping protein beta subunit family.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- 11 antibody
- Cap protein, actin, beta antibody
- Cap Z antibody
All lanes : Anti-CAPZB antibody (ab220669) at 1/100 dilution
Lane 1 : RT4 cell line lysate
Lane 2 : U251 MG cell line lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 31 kDa
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U251 MG cells labeling CAPZB with ab220669 at 4 µg/ml (green).
ab220669 has not yet been referenced specifically in any publications.