Product nameAnti-CAPZB antibody [EPR10236]
See all CAPZB primary antibodies
DescriptionRabbit monoclonal [EPR10236] to CAPZB
Tested applicationsSuitable for: IHC-P, WB, Flow Cyt, IPmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human CAPZB aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P47756
- HeLa, 293T, Jurkat and A431 cell lysates; Human kidney and placenta tissues; Jurkat cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab175212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/250.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 31 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/10 - 1/100.|
FunctionF-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.
Sequence similaritiesBelongs to the F-actin-capping protein beta subunit family.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- 11 antibody
- Cap protein, actin, beta antibody
- Cap Z antibody
All lanes : Anti-CAPZB antibody [EPR10236] (ab175212) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 31 kDa
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CAPZB with ab175212 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CAPZB with ab175212 at 1/100 dilution.
Flow cytometric analysis of permeabilized Jurkat cells labeling CAPZB with ab175212 at 1/10 dilution (red) compared to a Rabbit IgG negative control (green).
Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate using ab175212.
This product has been referenced in:
- Su X et al. Embryonic lethality in mice lacking Trim59 due to impaired gastrulation development. Cell Death Dis 9:302 (2018). Read more (PubMed: 29467473) »
- Sawhney S et al. Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a ?133p53a mimic. PLoS One 10:e0116270 (2015). WB ; Mouse . Read more (PubMed: 25643152) »