Overview

  • Product name
    Anti-CAPZB antibody [EPR10236]
    See all CAPZB primary antibodies
  • Description
    Rabbit monoclonal [EPR10236] to CAPZB
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cyt, IPmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CAPZB aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P47756

  • Positive control
    • HeLa, 293T, Jurkat and A431 cell lysates; Human kidney and placenta tissues; Jurkat cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab175212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.
    • Sequence similarities
      Belongs to the F-actin-capping protein beta subunit family.
    • Cellular localization
      Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • 11 antibody
      • Cap protein, actin, beta antibody
      • Cap Z antibody
      • CAPB antibody
      • CAPPB antibody
      • Capping protein (actin filament) muscle Z line beta antibody
      • Capping protein beta 2 antibody
      • CAPZ antibody
      • CapZ beta antibody
      • Capzb antibody
      • CAPZB_HUMAN antibody
      • DKFZp686K0524 antibody
      • F actin capping protein beta subunit antibody
      • F-actin-capping protein subunit beta antibody
      • FLJ12274 antibody
      • FLJ44693 antibody
      • MGC104401 antibody
      • MGC129749 antibody
      • MGC129750 antibody
      • wu:fk65f06 antibody
      • zgc:66149 antibody
      see all

    Images

    • All lanes : Anti-CAPZB antibody [EPR10236] (ab175212) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 31 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CAPZB with ab175212 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CAPZB with ab175212 at 1/100 dilution.

    • Flow cytometric analysis of permeabilized Jurkat cells labeling CAPZB with ab175212 at 1/10 dilution (red) compared to a Rabbit IgG negative control (green).

    • Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate using ab175212.

    References

    This product has been referenced in:
    • Su X  et al. Embryonic lethality in mice lacking Trim59 due to impaired gastrulation development. Cell Death Dis 9:302 (2018). Read more (PubMed: 29467473) »
    • Sawhney S  et al. Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a ?133p53a mimic. PLoS One 10:e0116270 (2015). WB ; Mouse . Read more (PubMed: 25643152) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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