Product nameCAPZB overexpression 293T lysate (whole cell)
ab94201 is a 293T cell transfected lysate in which Human CAPZB has been transiently over-expressed using a pCMV-CAPZB plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. Similarity: Belongs to the F-actin-capping protein beta subunit family.
Our Abpromise guarantee covers the use of ab94201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94201 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-CAPZB antibody (ab54930) at 1/500 dilution
Lane 1 :
CAPZB overexpression 293T lysate (whole cell) (ab94201)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94201 has not yet been referenced specifically in any publications.