Anti-Carbamyl-lysine antibody (ab175132)
Key features and details
- Rabbit polyclonal to Carbamyl-lysine
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Carbamyl-lysine antibody
See all Carbamyl-lysine primary antibodies -
Description
Rabbit polyclonal to Carbamyl-lysine -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Carbamyl-lysine conjugated to Keyhole Limpet Haemocyanin (KLH). The antiserum was prepared by the reaction of the protein with potassium cyanate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituent: 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/20000.
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| ELISA |
1/1000 - 1/20000.
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| Notes |
|---|
|
WB
1/1000 - 1/20000. |
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ELISA
1/1000 - 1/20000. |
Target
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Relevance
Carbamylation is a post-translational modification which occurs throughout the lifespan of proteins in vivo. Carbamylation results from the binding of isocyanic acid, spontaneously derived from high concentrations of urea and leading to the formation of carbamyl-lysine (CBL). The carbamylation of proteins is usually associated with a partial or complete loss of protein function. It is known that elevated urea directly induces the formation of potentially atherogenic carbamylated LDL (cLDL). High blood concentrations of urea leading to the carbamylation process were detected in uremic patients and patients with end-stage renal disease. -
Alternative names
- Carbamylation antibody
Datasheets and documents
References (2)
ab175132 has been referenced in 2 publications.
- Wang H et al. High urea induces depression and LTP impairment through mTOR signalling suppression caused by carbamylation. EBioMedicine 48:478-490 (2019). PubMed: 31628020
- Hutchinson D et al. Carbamylation/citrullination of IgG Fc in bronchiectasis, established RA with bronchiectasis and RA smokers: a potential risk factor for disease. ERJ Open Res 3:N/A (2017). PubMed: 29204430
