Key features and details
- Goat polyclonal to Carbonic Anhydrase 12/CA12
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Carbonic Anhydrase 12/CA12 antibody
See all Carbonic Anhydrase 12/CA12 primary antibodies
DescriptionGoat polyclonal to Carbonic Anhydrase 12/CA12
Specificityab223514 is expected to recognize all reported isoforms (NP_001209.1; NP_996808.1; NP_001280571.1).
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- WB: Human kidney lysate. IHC-P: Human kidney tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab223514 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab223514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).
1 hour primary incubation is recommended for this product.
FunctionReversible hydration of carbon dioxide.
Tissue specificityHighly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis.
Involvement in diseaseDefects in CA12 are the cause of hyperchlorhidrosis isolated (HCHLH) [MIM:143860]. HCHLH is a disorder characterized by excessive sweating and increased sweat chloride levels. Affected individuals suffer from episodes of hyponatremic dehydration and report increased amounts of visible salt precipitates in sweat.
Sequence similaritiesBelongs to the alpha-carbonic anhydrase family.
- Information by UniProt
- CA 12 antibody
- CA XII antibody
- CA-XII antibody
Paraffin-embedded human kidney tissue stained for CA12 using ab223514 at 5 µg/ml in immunohistochemical analysis, followed by AP-staining.
Anti-Carbonic Anhydrase 12/CA12 antibody (ab223514) at 0.3 µg/ml + human kidney lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 39 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
ab223514 has not yet been referenced specifically in any publications.