Overview

  • Product name

    Anti-Carbonic Anhydrase 12/CA12 antibody
    See all Carbonic Anhydrase 12/CA12 primary antibodies
  • Description

    Goat polyclonal to Carbonic Anhydrase 12/CA12
  • Host species

    Goat
  • Specificity

    ab223514 is expected to recognize all reported isoforms (NP_001209.1; NP_996808.1; NP_001280571.1).
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human Carbonic Anhydrase 12/CA12 aa 188-199 (internal sequence) (Cysteine residue). (NP_001209.1).
    Sequence:

    C-SHLQHVKYKGQE


    Database link: O43570

  • Positive control

    • WB: Human kidney lysate. IHC-P: Human kidney tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab223514 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).

Target

  • Function

    Reversible hydration of carbon dioxide.
  • Tissue specificity

    Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis.
  • Involvement in disease

    Defects in CA12 are the cause of hyperchlorhidrosis isolated (HCHLH) [MIM:143860]. HCHLH is a disorder characterized by excessive sweating and increased sweat chloride levels. Affected individuals suffer from episodes of hyponatremic dehydration and report increased amounts of visible salt precipitates in sweat.
  • Sequence similarities

    Belongs to the alpha-carbonic anhydrase family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CA 12 antibody
    • CA XII antibody
    • CA-XII antibody
    • CA12 antibody
    • CAH12_HUMAN antibody
    • Carbonate dehydratase XII antibody
    • Carbonic anhydrase 12 antibody
    • Carbonic anhydrase XII antibody
    • Carbonic dehydratase antibody
    • CAXII antibody
    • FLJ20151 antibody
    • HsT18816 antibody
    • T18816 antibody
    • Tumor antigen HOM RCC 3.1.3 antibody
    • Tumor antigen HOM-RCC-3.1.3 antibody
    see all

Images

  • Paraffin-embedded human kidney tissue stained for CA12 using ab223514 at 5 µg/ml in immunohistochemical analysis, followed by AP-staining.

  • Anti-Carbonic Anhydrase 12/CA12 antibody (ab223514) at 0.3 µg/ml + human kidney lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 39 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab223514 has not yet been referenced specifically in any publications.

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