Overview

  • Product name

    Anti-Carbonic Anhydrase 12/CA12 antibody
    See all Carbonic Anhydrase 12/CA12 primary antibodies
  • Description

    Rabbit polyclonal to Carbonic Anhydrase 12/CA12
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Carbonic Anhydrase 12/CA12 aa 231-280. The exact sequence is proprietary.
    Database link: O43570

  • Positive control

    • IHC-P: Human kidney and colon tissues. WB: MCF7 whole cell extract.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol

    (PBS without Mg2+ and Ca2+).
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab230515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 39 kDa.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Reversible hydration of carbon dioxide.
  • Tissue specificity

    Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis.
  • Involvement in disease

    Defects in CA12 are the cause of hyperchlorhidrosis isolated (HCHLH) [MIM:143860]. HCHLH is a disorder characterized by excessive sweating and increased sweat chloride levels. Affected individuals suffer from episodes of hyponatremic dehydration and report increased amounts of visible salt precipitates in sweat.
  • Sequence similarities

    Belongs to the alpha-carbonic anhydrase family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CA 12 antibody
    • CA XII antibody
    • CA-XII antibody
    • CA12 antibody
    • CAH12_HUMAN antibody
    • Carbonate dehydratase XII antibody
    • Carbonic anhydrase 12 antibody
    • Carbonic anhydrase XII antibody
    • Carbonic dehydratase antibody
    • CAXII antibody
    • FLJ20151 antibody
    • HsT18816 antibody
    • T18816 antibody
    • Tumor antigen HOM RCC 3.1.3 antibody
    • Tumor antigen HOM-RCC-3.1.3 antibody
    see all

Images

  • All lanes : Anti-Carbonic Anhydrase 12/CA12 antibody (ab230515) at 1/500 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell extract
    Lane 2 : MCF7 (human breast adenocarcinoma cell line) cell extract with synthesized peptide

    Predicted band size: 39 kDa

  • Formalin-fixed, paraffin-embedded human kidney tissue stained for CA12 using ab230515 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon tissue stained for CA12 using ab230515 at 1/50 dilution in immunohistochemical analysis.

References

ab230515 has not yet been referenced specifically in any publications.

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