Key features and details
- Rabbit polyclonal to Carbonic Anhydrase 12/CA12
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Carbonic Anhydrase 12/CA12 antibody
See all Carbonic Anhydrase 12/CA12 primary antibodies
DescriptionRabbit polyclonal to Carbonic Anhydrase 12/CA12
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human Carbonic Anhydrase 12/CA12 aa 231-280. The exact sequence is proprietary.
Database link: O43570
- IHC-P: Human kidney and colon tissues. WB: MCF7 whole cell extract.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol
(PBS without Mg2+ and Ca2+).
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 39 kDa.|
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionReversible hydration of carbon dioxide.
Tissue specificityHighly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis.
Involvement in diseaseDefects in CA12 are the cause of hyperchlorhidrosis isolated (HCHLH) [MIM:143860]. HCHLH is a disorder characterized by excessive sweating and increased sweat chloride levels. Affected individuals suffer from episodes of hyponatremic dehydration and report increased amounts of visible salt precipitates in sweat.
Sequence similaritiesBelongs to the alpha-carbonic anhydrase family.
- Information by UniProt
- CA 12 antibody
- CA XII antibody
- CA-XII antibody
All lanes : Anti-Carbonic Anhydrase 12/CA12 antibody (ab230515) at 1/500 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell extract
Lane 2 : MCF7 (human breast adenocarcinoma cell line) cell extract with synthesized peptide
Predicted band size: 39 kDa
Formalin-fixed, paraffin-embedded human kidney tissue stained for CA12 using ab230515 at 1/50 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human colon tissue stained for CA12 using ab230515 at 1/50 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230515 has been referenced in 1 publication.
- Gu XF et al. Prognostic value of carbonic anhydrase XII (CA XII) overexpression in hepatocellular carcinoma. Int J Clin Exp Pathol 12:2173-2183 (2019). PubMed: 31934040