Key features and details
- Rabbit polyclonal to Carbonic Anhydrase 12/CA12
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Carbonic Anhydrase 12/CA12 antibody
See all Carbonic Anhydrase 12/CA12 primary antibodies
DescriptionRabbit polyclonal to Carbonic Anhydrase 12/CA12
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- IHC-P: Human kidney tissue. ICC/IF: RT4 cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab244309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionReversible hydration of carbon dioxide.
Tissue specificityHighly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis.
Involvement in diseaseDefects in CA12 are the cause of hyperchlorhidrosis isolated (HCHLH) [MIM:143860]. HCHLH is a disorder characterized by excessive sweating and increased sweat chloride levels. Affected individuals suffer from episodes of hyponatremic dehydration and report increased amounts of visible salt precipitates in sweat.
Sequence similaritiesBelongs to the alpha-carbonic anhydrase family.
- Information by UniProt
- CA 12 antibody
- CA XII antibody
- CA-XII antibody
Formalin-fixed, paraffin-embedded human kidney tissue stained for Carbonic Anhydrase 12/CA12 using ab244309 at 1/200 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized RT4 (human urinary bladder cancer cell line) cells stained for Carbonic Anhydrase 12/CA12 (green) using ab244309 at 4 µg/ml in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244309 has not yet been referenced specifically in any publications.