Recombinant
RabMAb

Recombinant Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - BSA and Azide free (ab236138)

Overview

  • Product name

    Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - BSA and Azide free
    See all Carbonic Anhydrase 12/CA12 primary antibodies
  • Description

    Rabbit monoclonal [EPR14861] to Carbonic Anhydrase 12/CA12 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Carbonic Anhydrase 12/CA12 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: O43570

  • Positive control

    • IHC-P: Human colon tissue.
  • General notes

    Ab236138 is the carrier-free version of ab195233. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236138 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14861
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).

Target

  • Function

    Reversible hydration of carbon dioxide.
  • Tissue specificity

    Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis.
  • Involvement in disease

    Defects in CA12 are the cause of hyperchlorhidrosis isolated (HCHLH) [MIM:143860]. HCHLH is a disorder characterized by excessive sweating and increased sweat chloride levels. Affected individuals suffer from episodes of hyponatremic dehydration and report increased amounts of visible salt precipitates in sweat.
  • Sequence similarities

    Belongs to the alpha-carbonic anhydrase family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CA 12 antibody
    • CA XII antibody
    • CA-XII antibody
    • CA12 antibody
    • CAH12_HUMAN antibody
    • Carbonate dehydratase XII antibody
    • Carbonic anhydrase 12 antibody
    • Carbonic anhydrase XII antibody
    • Carbonic dehydratase antibody
    • CAXII antibody
    • FLJ20151 antibody
    • HsT18816 antibody
    • T18816 antibody
    • Tumor antigen HOM RCC 3.1.3 antibody
    • Tumor antigen HOM-RCC-3.1.3 antibody
    see all

Images

  • Flow cytometric analysis of SK-OV-3 cells (paraformaldehyde-fixed, 2%) labeling CA12 with ab195233 at 1/130 dilution (red) or a Rabbit monoclonal IgG (negative) (black), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. Unlabeled cells (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195233).

  • Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

    Note: Cell membrane and cytoplasm staining on human stomach tissue was observed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195233).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

    Note: Cell membrane and weakly cytoplasm staining on human pancreas tissue was observed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195233).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

    Note: Cell membrane and weakly cytoplasm staining on human transitional cell carcinoma of bladder tissue was observed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195233).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling CA12 with ab195233 at 1/100  dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

    Note: Cell membrane and weakly cytoplasm staining on human ovarian carcinoma tissue was observed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195233).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human renal adenocarcinoma tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

    Note: Cell membrane and cytoplasm staining on human renal adenocarcinoma tissue was observed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195233).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

    Note: Cell membrane and cytoplasm staining on human colon tissue was observed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195233).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab236138 has not yet been referenced specifically in any publications.

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