RecombinantRabMAb

Recombinant Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14861] to Carbonic Anhydrase 12/CA12 - C-terminal
  • Suitable for: Flow Cyt, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal
    See all Carbonic Anhydrase 12/CA12 primary antibodies

  • Description

    Rabbit monoclonal [EPR14861] to Carbonic Anhydrase 12/CA12 - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Carbonic Anhydrase 12/CA12 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: O43570

  • Positive control

    • MCF7, SKOV3, 293, HeLa, A549, Human fetal kidney, Human ovary cancer and Human breast cancer lysates; Human renal adenocarcinoma, ovarian carcinoma, bladder transitional cell carcinoma, pancreas, colon and stomach tissues; SK-OV-3 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14861

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab195233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/130.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).

Target

  • Function

    Reversible hydration of carbon dioxide.

  • Tissue specificity

    Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis.

  • Involvement in disease

    Defects in CA12 are the cause of hyperchlorhidrosis isolated (HCHLH) [MIM:143860]. HCHLH is a disorder characterized by excessive sweating and increased sweat chloride levels. Affected individuals suffer from episodes of hyponatremic dehydration and report increased amounts of visible salt precipitates in sweat.

  • Sequence similarities

    Belongs to the alpha-carbonic anhydrase family.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CA 12 antibody
      • CA XII antibody
      • CA-XII antibody
      • CA12 antibody
      • CAH12_HUMAN antibody
      • Carbonate dehydratase XII antibody
      • Carbonic anhydrase 12 antibody
      • Carbonic anhydrase XII antibody
      • Carbonic dehydratase antibody
      • CAXII antibody
      • FLJ20151 antibody
      • HsT18816 antibody
      • T18816 antibody
      • Tumor antigen HOM RCC 3.1.3 antibody
      • Tumor antigen HOM-RCC-3.1.3 antibody
      see all

    Images

    • Western blot - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
      Western blot - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
      All lanes : Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233) at 1/10000 dilution

      Lane 1 : MCF7 cell lysate
      Lanes 2 & 5 : SKOV3 cell lysate
      Lane 3 : 293 cell lysate
      Lane 4 : HeLa cell lysate
      Lane 6 : Human fetal kidney lysate
      Lane 7 : Human ovary cancer lysate
      Lane 8 : Human breast cancer lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 39 kDa


      Exposure time: 10 seconds
    • Flow Cytometry - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
      Flow Cytometry - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)

      Flow cytometric analysis of SK-OV-3 cells (paraformaldehyde-fixed, 2%) labeling CA12 with ab195233 at 1/130 dilution (red) or a Rabbit monoclonal IgG (negative) (black), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. Unlabeled cells (Blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)

      Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

      Note: Cell membrane and cytoplasm staining on human stomach tissue was observed.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)

      Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

      Note: Cell membrane and cytoplasm staining on human colon tissue was observed.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)

      Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

      Note: Cell membrane and weakly cytoplasm staining on human pancreas tissue was observed.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)

      Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

      Note: Cell membrane and weakly cytoplasm staining on human transitional cell carcinoma of bladder tissue was observed.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)

      Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling CA12 with ab195233 at 1/100  dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

      Note: Cell membrane and weakly cytoplasm staining on human ovarian carcinoma tissue was observed.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)

      Immunohistochemical analysis of paraffin-embedded Human renal adenocarcinoma tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

      Note: Cell membrane and cytoplasm staining on human renal adenocarcinoma tissue was observed.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
      Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)

    References (0)

    Publishing research using ab195233? Please let us know so that we can cite the reference in this datasheet.

    ab195233 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CA12 antibody [EPR14861] - C-terminal

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 03 2018

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