Recombinant Anti-Carbonic Anhydrase 14/CA14 antibody [EPR14307] - BSA and Azide free (ab250734)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14307] to Carbonic Anhydrase 14/CA14 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Carbonic Anhydrase 14/CA14 antibody [EPR14307] - BSA and Azide free
See all Carbonic Anhydrase 14/CA14 primary antibodies -
Description
Rabbit monoclonal [EPR14307] to Carbonic Anhydrase 14/CA14 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab250734 is the carrier-free version of ab184180.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR14307 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa).
|
Notes |
---|
WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa). |
Target
-
Function
Reversible hydration of carbon dioxide. -
Tissue specificity
High expression in all parts of the central nervous system and lower expression in adult liver, heart, small intestine, colon, kidney, urinary bladder and skeletal muscle. -
Sequence similarities
Belongs to the alpha-carbonic anhydrase family. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 23632 Human
- Entrez Gene: 23831 Mouse
- Entrez Gene: 791259 Rat
- Omim: 604832 Human
- SwissProt: Q9ULX7 Human
- SwissProt: Q9WVT6 Mouse
- Unigene: 528988 Human
- Unigene: 489647 Mouse
-
Alternative names
- CA 14 antibody
- CA XIV antibody
- CA-XIV antibody
see all
Images
-
All lanes : Anti-Carbonic Anhydrase 14/CA14 antibody [EPR14307] (ab184180) at 1/5000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : U87-MG cell lysate
Lane 3 : A375 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?This data was developed using ab184180, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab250734 has not yet been referenced specifically in any publications.