Key features and details
- Rabbit polyclonal to Carbonic anhydrase 2/CA2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Carbonic anhydrase 2/CA2 antibody
See all Carbonic anhydrase 2/CA2 primary antibodies
DescriptionRabbit polyclonal to Carbonic anhydrase 2/CA2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
This product is the 100 µg version of ab6621.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.
Our Abpromise guarantee covers the use of ab191343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
|IHC-P||Use at an assay dependent concentration.|
FunctionEssential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye.
Involvement in diseaseDefects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3) [MIM:259730]; also known as osteopetrosis with renal tubular acidosis, carbonic anhydrase II deficiency syndrome, Guibaud-Vainsel syndrome or marble brain disease. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB3 is associated with renal tubular acidosis, cerebral calcification (marble brain disease) and in some cases with mental retardation.
Sequence similaritiesBelongs to the alpha-carbonic anhydrase family.
- Information by UniProt
- CA 2 antibody
- CA II antibody
- CA-II antibody
Anti-Carbonic anhydrase 2/CA2 antibody (ab191343) at 1/1000 dilution + Carbonic Anhydrase II at 0.05 µg
Rabbit Peroxidase at 1/40000 dilution
Formalin-fixed, paraffin-embedded human kidney tissue stained for Carbonic anhydrase 2/CA2 using ab191343 at 4 μg/ml in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse eye tissue stained for Carbonic anhydrase 2/CA2 using ab191343 at 4 μg/ml in immunohistochemical analysis.
ab191343 has been referenced in 13 publications.
- Bamba R et al. Evaluation of a Nerve Fusion Technique With Polyethylene Glycol in a Delayed Setting After Nerve Injury. J Hand Surg Am 43:82.e1-82.e7 (2018). IHC-P . PubMed: 28823535
- Harter TS et al. A solution to Nature's haemoglobin knockout: a plasma-accessible carbonic anhydrase catalyses CO2 excretion in Antarctic icefish gills. J Exp Biol 221:N/A (2018). PubMed: 30291156
- Fehsenfeld S & Wood CM Section-specific expression of acid-base and ammonia transporters in the kidney tubules of the goldfish Carassius auratus and their responses to feeding. Am J Physiol Renal Physiol 315:F1565-F1582 (2018). PubMed: 30089033
- Tachibana H et al. Carbonic anhydrase 2 is a novel invasion-associated factor in urinary bladder cancers. Cancer Sci 108:331-337 (2017). PubMed: 28004470
- Pandya M et al. Integrative Temporo-Spatial, Mineralogic, Spectroscopic, and Proteomic Analysis of Postnatal Enamel Development in Teeth with Limited Growth. Front Physiol 8:793 (2017). PubMed: 29114228