Anti-Carbonic anhydrase 2/CA2 antibody (ab231153)
Key features and details
- Rabbit polyclonal to Carbonic anhydrase 2/CA2
- Suitable for: WB
- Reacts with: Chicken, Human
- Isotype: IgG
Overview
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Product name
Anti-Carbonic anhydrase 2/CA2 antibody
See all Carbonic anhydrase 2/CA2 primary antibodies -
Description
Rabbit polyclonal to Carbonic anhydrase 2/CA2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Chicken, Human -
Immunogen
Recombinant full length protein (His-tag) corresponding to Chicken Carbonic anhydrase 2/CA2 aa 1 to the C-terminus. Expressed in E. coli.
Database link: P07630 -
Positive control
- WB: Recombinant chicken Carbonic anhydrase 2/CA2 protein. HEK-293T cell lysate; Chicken testis and eye lysate; Chicken serum.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 29 kDa.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 29 kDa. |
Target
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Function
Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. -
Involvement in disease
Defects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3) [MIM:259730]; also known as osteopetrosis with renal tubular acidosis, carbonic anhydrase II deficiency syndrome, Guibaud-Vainsel syndrome or marble brain disease. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB3 is associated with renal tubular acidosis, cerebral calcification (marble brain disease) and in some cases with mental retardation. -
Sequence similarities
Belongs to the alpha-carbonic anhydrase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 760 Human
- Omim: 611492 Human
- SwissProt: P00918 Human
- Unigene: 155097 Human
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Alternative names
- CA 2 antibody
- CA II antibody
- CA-II antibody
see all
Images
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Anti-Carbonic anhydrase 2/CA2 antibody (ab231153) at 2 µg/ml + Chicken serum
Predicted band size: 29 kDa -
Anti-Carbonic anhydrase 2/CA2 antibody (ab231153) at 2 µg/ml + HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Predicted band size: 29 kDa -
Anti-Carbonic anhydrase 2/CA2 antibody (ab231153) at 1 µg/ml + Chicken eye lysate
Predicted band size: 29 kDa -
Anti-Carbonic anhydrase 2/CA2 antibody (ab231153) at 2 µg/ml + Chicken testis lysate
Predicted band size: 29 kDa -
Anti-Carbonic anhydrase 2/CA2 antibody (ab231153) at 2 µg/ml + Recombinant chicken Carbonic anhydrase 2 protein
Predicted band size: 29 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231153 has not yet been referenced specifically in any publications.