Key features and details
- Rabbit polyclonal to Carbonic anhydrase 2/CA2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Carbonic anhydrase 2/CA2 antibody
See all Carbonic anhydrase 2/CA2 primary antibodies
DescriptionRabbit polyclonal to Carbonic anhydrase 2/CA2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Sheep, Rabbit, Cow
Synthetic peptide corresponding to Human Carbonic anhydrase 2/CA2 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- WB: HAP1, HEK293T, A431 and Caco-2 cell lysates; Human bone marrow, small intestine and kidney tissue lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
KO cell lines
KO cell lysates
Our Abpromise guarantee covers the use of ab82559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).|
FunctionEssential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye.
Involvement in diseaseDefects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3) [MIM:259730]; also known as osteopetrosis with renal tubular acidosis, carbonic anhydrase II deficiency syndrome, Guibaud-Vainsel syndrome or marble brain disease. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB3 is associated with renal tubular acidosis, cerebral calcification (marble brain disease) and in some cases with mental retardation.
Sequence similaritiesBelongs to the alpha-carbonic anhydrase family.
- Information by UniProt
- CA 2 antibody
- CA II antibody
- CA-II antibody
All lanes : Anti-Carbonic anhydrase 2/CA2 antibody (ab82559) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : CA2 knockout HEK293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Lanes 1-4: Merged signal (red and green). Green - ab82559 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.
ab82559 Anti-Carbonic anhydrase 2/CA2 antibody was shown to specifically react with Carbonic anhydrase 2/CA2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab265072 (knockout cell lysate ab257084) was used. Wild-type and Carbonic anhydrase 2/CA2 knockout samples were subjected to SDS-PAGE. ab82559 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Carbonic Anhydrase II knockout HAP1 cell lysate (20 µg)
Lane 3: A431 cell lysate (20 µg)
Lane 4: HEk293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab82559 observed at 30 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab82559 was shown to specifically react with Carbonic Anhydrase II when Carbonic Anhydrase II knockout samples were used. Wild-type and Carbonic Anhydrase II knockout samples were subjected to SDS-PAGE. ab82559 and ab8245 (loading control to GAPDH) were diluted at 1 μg/mL and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-Carbonic anhydrase 2/CA2 antibody (ab82559) at 1 µg/ml
Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 1 minute
ab82559 has been referenced in 1 publication.
- Roossink F et al. The role of ATM and 53BP1 as predictive markers in cervical cancer. Int J Cancer 131:2056-66 (2012). PubMed: 22323184