Recombinant Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (ab124687)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5195] to Carbonic anhydrase 2/CA2
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195]
See all Carbonic anhydrase 2/CA2 primary antibodies -
Description
Rabbit monoclonal [EPR5195] to Carbonic anhydrase 2/CA2 -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide corresponding to Human Carbonic anhydrase 2/CA2 aa 200-300 (C terminal).
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Positive control
- WB: A431, Caco 2 and HEK293T cell lysates; Human heart and fetal brain lysates. IHC-P: Human kidney and brain tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.42 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5195 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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KO cell pellets
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IHC-P |
Human
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WB |
Human
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All applications |
Mouse
Rat
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Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. -
Involvement in disease
Defects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3) [MIM:259730]; also known as osteopetrosis with renal tubular acidosis, carbonic anhydrase II deficiency syndrome, Guibaud-Vainsel syndrome or marble brain disease. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB3 is associated with renal tubular acidosis, cerebral calcification (marble brain disease) and in some cases with mental retardation. -
Sequence similarities
Belongs to the alpha-carbonic anhydrase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 760 Human
- Entrez Gene: 12349 Mouse
- Entrez Gene: 54231 Rat
- Omim: 611492 Human
- SwissProt: P00918 Human
- SwissProt: P00920 Mouse
- SwissProt: P27139 Rat
- Unigene: 155097 Human
see all -
Alternative names
- CA 2 antibody
- CA II antibody
- CA-II antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (ab124687)
ab124687, at 1/250 dilution, staining Carbonic anhydrase 2/CA2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (ab124687) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : CA2 knockout HEK293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDaLanes 1-4: Merged signal (red and green). Green - ab124687 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.
ab124687 Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] was shown to specifically react with Carbonic anhydrase 2/CA2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab265072 (knockout cell lysate ab257084) was used. Wild-type and Carbonic anhydrase 2/CA2 knockout samples were subjected to SDS-PAGE. ab124687 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: Carbonic anhydrase 2/CA2 knockout HAP1 cell lysate (40 µg)
Lane 3: A431 cell lysate (40 µg)
Lane 4: HEK293 cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab124687 observed at 32 kDa. Red - loading control, ab8245, observed at 37 kDa.ab124687 was shown to specifically react with Carbonic anhydrase 2/CA2 when Carbonic anhydrase 2/CA2 knockout samples were used. Wild-type and Carbonic anhydrase 2/CA2 knockout samples were subjected to SDS-PAGE. Ab124687 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (ab124687) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Caco 2 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Human heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (ab124687)
ab124687, at 1/250 dilution, staining Carbonic anhydrase 2/CA2 in paraffin-embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab124687 has been referenced in 12 publications.
- Li S et al. PKC-d deficiency in B cells displays osteopenia accompanied with upregulation of RANKL expression and osteoclast-osteoblast uncoupling. Cell Death Dis 11:762 (2020). PubMed: 32938907
- Hannen R et al. Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor. Cancers (Basel) 11:N/A (2019). PubMed: 31262047
- Suri BK et al. Carbonic anhydrases in human keratinocytes and their regulation by all-trans retinoic acid and 1a,25-dihydroxyvitamin D3. Exp Dermatol 28:976-980 (2019). PubMed: 31125456
- Li H et al. Expression and localization of Forkhead transcription factor A1 in the three-dimensional reconstructed eccrine sweat glands. Acta Histochem 120:520-524 (2018). PubMed: 29909922
- Suri BK et al. Toll-like Receptor 3 Agonist, Polyinosinic-polycytidylic Acid, Upregulates Carbonic Anhydrase II in Human Keratinocytes. Acta Derm Venereol 98:762-765 (2018). PubMed: 29738048