Key features and details
- Rabbit polyclonal to Carbonic Anhydrase 3/CA3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human, Recombinant fragment
- Isotype: IgG
Product nameAnti-Carbonic Anhydrase 3/CA3 antibody
See all Carbonic Anhydrase 3/CA3 primary antibodies
DescriptionRabbit polyclonal to Carbonic Anhydrase 3/CA3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human, Recombinant fragment
Predicted to work with: Chimpanzee, Macaque monkey, Chinese hamster, Orangutan
Synthetic peptide corresponding to Human Carbonic Anhydrase 3/CA3 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Recombinant Carbonic anhydrase 3 protein as well as the following tissue lysates: Human Skeletal Muscle; Mouse Skeletal Muscle; Rat Skeletal Muscle. It also gave a positive result in FFPE human skeletal muscle tissue sections.
This product was previously labelled as Carbonic Anhydrase III
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab150037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 29 kDa).|
|IHC-P||Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionReversible hydration of carbon dioxide.
Tissue specificityMuscle specific.
Sequence similaritiesBelongs to the alpha-carbonic anhydrase family.
Developmental stageAt 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.
modificationsS-glutathionylated in hepatocytes under oxidative stress.
- Information by UniProt
- CA III antibody
- CA-III antibody
- Ca3 antibody
All lanes : Anti-Carbonic Anhydrase 3/CA3 antibody (ab150037) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 3 : Skeletal Muscle (Rat) Tissue Lysate
Lane 4 : Carbonic Anhydrase III/CA3 protein (ab50779)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Additional bands at: 24 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
IHC image of Carbonic Anydrase III staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab150037, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab150037 has not yet been referenced specifically in any publications.