Overview

  • Product name

    Anti-Carbonic Anhydrase 3/CA3 antibody
    See all Carbonic Anhydrase 3/CA3 primary antibodies
  • Description

    Rabbit polyclonal to Carbonic Anhydrase 3/CA3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Recombinant fragment
    Predicted to work with: Chimpanzee, Macaque monkey, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Carbonic Anhydrase 3/CA3 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab136233)

  • Positive control

    • This antibody gave a positive signal in Recombinant Carbonic anhydrase 3 protein as well as the following tissue lysates: Human Skeletal Muscle; Mouse Skeletal Muscle; Rat Skeletal Muscle. It also gave a positive result in FFPE human skeletal muscle tissue sections.
  • General notes

     This product was previously labelled as Carbonic Anhydrase III

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 29 kDa).
IHC-P Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Reversible hydration of carbon dioxide.
  • Tissue specificity

    Muscle specific.
  • Sequence similarities

    Belongs to the alpha-carbonic anhydrase family.
  • Developmental stage

    At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.
  • Post-translational
    modifications

    S-glutathionylated in hepatocytes under oxidative stress.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • CA III antibody
    • CA-III antibody
    • Ca3 antibody
    • CAH3_HUMAN antibody
    • CAIII antibody
    • Car3 antibody
    • Carbonate dehydratase III antibody
    • Carbonic anhydrase 3 antibody
    • Carbonic anhydrase III antibody
    • Carbonic anhydrase III muscle specific antibody
    • EC 4.2.1.1 antibody
    • Epididymis secretory sperm binding protein Li 167mP antibody
    • HEL S 167mP antibody
    see all

Images

  • All lanes : Anti-Carbonic Anhydrase 3/CA3 antibody (ab150037) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 3 : Skeletal Muscle (Rat) Tissue Lysate
    Lane 4 : Carbonic Anhydrase III/CA3 protein (ab50779)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 24 kDa, 58 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds
  • IHC image of Carbonic Anydrase III staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab150037, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

ab150037 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

The lab confirmed that this lot is BSA-free. Please let me know if you have any other questions.

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