Key features and details
- Rabbit polyclonal to Carbonic Anhydrase 3/CA3
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Carbonic Anhydrase 3/CA3 antibody
See all Carbonic Anhydrase 3/CA3 primary antibodies
DescriptionRabbit polyclonal to Carbonic Anhydrase 3/CA3
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Horse, Pig
Recombinant full length protein corresponding to Human Carbonic Anhydrase 3/CA3 aa 1-260.
MAKEWGYASHNGPDHWHELFPNAKGENQSPVELHTKDIRHDPSLQPWSVS YDGGSAKTILNNGKTCRVVFDDTYDRSMLRGGPLPGPYRLRQFHLHWGSS DDHGSEHTVDGVKYAAELHLVHWNPKYNTFKEALKQRDGIAVIGIFLKIG HENGEFQIFLDALDKIKTKGKEAPFTKFDPSCLFPACRDYWTYQGSFTTP PCEECIVWLLLKEPMTVSSDQMAKLRSLLSSAENEPPVPLVSNWRPPQPI NNRVVRASFK
Database link: P07451
- Recombinant Human Carbonic Anhydrase 3/CA3 protein (BSA and azide free) (ab173078) can be used as a positive control in WB. HeLa and 293 cell lysate.
This product was previously labelled as Carbonic Anhydrase III
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab175309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/500 - 1/2000. Predicted molecular weight: 30 kDa.|
FunctionReversible hydration of carbon dioxide.
Tissue specificityMuscle specific.
Sequence similaritiesBelongs to the alpha-carbonic anhydrase family.
Developmental stageAt 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.
modificationsS-glutathionylated in hepatocytes under oxidative stress.
- Information by UniProt
- CA III antibody
- CA-III antibody
- Ca3 antibody
All lanes : Anti-Carbonic Anhydrase 3/CA3 antibody (ab175309) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Predicted band size: 30 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue labelling Carbonic Anhydrase 3/CA3 with ab175309 at 1/200. Magnification: 200x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue labelling Carbonic Anhydrase 3/CA3 with ab175309 at 1/200. Magnification: 400x.
Immunocytochemistry/Immunofluorescence analysis of HeLa cells using ab175309. Blue DAPI for nuclear staining.
ab175309 has not yet been referenced specifically in any publications.