Anti-Carbonic Anhydrase 3/CA3 antibody (ab196835)
Key features and details
- Rabbit polyclonal to Carbonic Anhydrase 3/CA3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Carbonic Anhydrase 3/CA3 antibody
See all Carbonic Anhydrase 3/CA3 primary antibodies -
Description
Rabbit polyclonal to Carbonic Anhydrase 3/CA3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Horse, Cow, Pig -
Immunogen
Recombinant full length protein corresponding to Human Carbonic Anhydrase 3/CA3 aa 1 to the C-terminus.
Database link: P07451 -
Positive control
- Skeletal Muscle (Mouse) Whole Cell Lysate (ab3869) can be used as a positive control in WB. Mouse kidney, lung and skeletal muscle extracts; Rat testis tissue; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS without Mg2+ and Ca2+. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50 - 1/100.
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WB |
1/500 - 1/2000. Predicted molecular weight: 30 kDa.
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ICC/IF |
1/50 - 1/200.
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Notes |
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IHC-P
1/50 - 1/100. |
WB
1/500 - 1/2000. Predicted molecular weight: 30 kDa. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Reversible hydration of carbon dioxide. -
Tissue specificity
Muscle specific. -
Sequence similarities
Belongs to the alpha-carbonic anhydrase family. -
Developmental stage
At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord. -
Post-translational
modificationsS-glutathionylated in hepatocytes under oxidative stress. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 513212 Cow
- Entrez Gene: 100050125 Horse
- Entrez Gene: 761 Human
- Entrez Gene: 12350 Mouse
- Entrez Gene: 494016 Pig
- Entrez Gene: 54232 Rat
- Omim: 114750 Human
- SwissProt: Q3SZX4 Cow
see all -
Alternative names
- CA III antibody
- CA-III antibody
- Ca3 antibody
see all
Images
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All lanes : Anti-Carbonic Anhydrase 3/CA3 antibody (ab196835) at 1/500 dilution
Lane 1 : Mouse kidney extract
Lane 2 : Mouse lung extract
Lane 3 : Mouse skeletal muscle extract
Predicted band size: 30 kDa -
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Carbonic Anhydrase 3/CA3 with ab196835 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Carbonic Anhydrase 3/CA3 with ab196835 at 1/200 dilution.
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Immunofluorescent analysis of HeLa cells labeling Carbonic Anhydrase III/CA3 with ab196835 at 1/50 dilution. Blue: DAPI for nuclear staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab196835 has been referenced in 2 publications.
- Sinkemani A et al. Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions. Stem Cells Int 2020:8882549 (2020). PubMed: 33424982
- Huang H et al. CAIII expression in skeletal muscle is regulated by Ca2+-CaMKII-MEF2C signaling. Exp Cell Res 385:111672 (2019). PubMed: 31614133