Product nameAnti-Carbonic Anhydrase 3/CA3 antibody [EPR13425]
See all Carbonic Anhydrase 3/CA3 primary antibodies
DescriptionRabbit monoclonal [EPR13425] to Carbonic Anhydrase 3/CA3
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Carbonic Anhydrase 3/CA3 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P07451
- Human skeletal muscle, rat muscle, and mouse muscle lysates, Human skeletal muscle tissue
This product was previously labelled as Carbonic Anhydrase III
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 29 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionReversible hydration of carbon dioxide.
Tissue specificityMuscle specific.
Sequence similaritiesBelongs to the alpha-carbonic anhydrase family.
Developmental stageAt 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.
modificationsS-glutathionylated in hepatocytes under oxidative stress.
- Information by UniProt
- CA III antibody
- CA-III antibody
- Ca3 antibody
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Carbonic Anhydrase 3/CA3 with ab181358 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-Carbonic Anhydrase 3/CA3 antibody [EPR13425] (ab181358) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysates
Lane 2 : Rat muscle lysates
Lane 3 : Mouse muscle lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 29 kDa