Anti-Carbonic Anhydrase 4/CA4 antibody [C1] (ab239505)
Key features and details
- Mouse monoclonal [C1] to Carbonic Anhydrase 4/CA4
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG2b
Overview
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Product name
Anti-Carbonic Anhydrase 4/CA4 antibody [C1]
See all Carbonic Anhydrase 4/CA4 primary antibodies -
Description
Mouse monoclonal [C1] to Carbonic Anhydrase 4/CA4 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Rat Carbonic Anhydrase 4/CA4 aa 1-250. Two N-terminal tags, His-tag and T7-tag (Expressed in E.coli).
Database link: P48284 -
Positive control
- IHC-P: Rat skeletal muscle tissue. WB: Rat lung and brain lysates; Recombinant rat Carbonic Anhydrase IV/CA4 protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
C1 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab239505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 30 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 35 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 30 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 35 kDa. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 29242 Rat
- SwissProt: P48284 Rat
- Unigene: 51389 Rat
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Alternative names
- CA IV antibody
- CA4 antibody
- CAH4_HUMAN antibody
see all
Images
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Paraffin-embedded rat skeletal muscle tissue stained for Carbonic Anhydrase 4/CA4 using ab239505 at 30 µg/ml in immunohistochemical analysis. DAB staining.
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All lanes : Anti-Carbonic Anhydrase 4/CA4 antibody [C1] (ab239505) at 2 µg/ml
Lane 1 : Rat lung lysate
Lane 2 : Rat brain lysate
Secondary
All lanes : HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution
Predicted band size: 35 kDa -
Anti-Carbonic Anhydrase 4/CA4 antibody [C1] (ab239505) at 2 µg/ml + Recombinant rat Carbonic Anhydrase IV/CA4 protein
Secondary
HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution
Predicted band size: 35 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab239505 has been referenced in 1 publication.
- Yuan Y et al. A Pulmonary Vascular Model From Endothelialized Whole Organ Scaffolds. Front Bioeng Biotechnol 9:760309 (2021). PubMed: 34869270