Key features and details
- Mouse monoclonal [C1] to Carbonic Anhydrase 4/CA4
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG2b
Product nameAnti-Carbonic Anhydrase 4/CA4 antibody [C1]
See all Carbonic Anhydrase 4/CA4 primary antibodies
DescriptionMouse monoclonal [C1] to Carbonic Anhydrase 4/CA4
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat
Recombinant fragment (His-T7-tag) corresponding to Rat Carbonic Anhydrase 4/CA4 aa 20-220. Two N-terminal tags, His-tag and T7-tag (Expressed in E.coli).
SHWCYEIQAKEPNSHCSGPEQWTGDCKKNQQSPINIVTSKTKLNPSLTPF TFVGYDQKKKWEVKNNQHSVEMSLGEDIYIFGGDLPTQYKAIQLHLHWSE ESNKGSEHSIDGKHFAMEMHVVHKKMTTGDKVQDSDSKDKIAVLAFMVEV GNEVNEGFQPLVEALSRLSKPFTNSTVSESCLQDMLPEKKKLSAYFRYQG S
Database link: P48284
- IHC-P: Rat skeletal muscle tissue. WB: Rat lung and brain lysates; Recombinant rat Carbonic Anhydrase IV/CA4 protein.
This product was previously labelled as Carbonic Anhydrase IV
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from TCS.
Light chain typekappa
Our Abpromise guarantee covers the use of ab239505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 30 µg/ml.|
|WB||Use a concentration of 0.5 - 5 mg/ml. Predicted molecular weight: 35 kDa.|
- Information by UniProt
- CA IV antibody
- CA4 antibody
- CAH4_HUMAN antibody
Paraffin-embedded rat skeletal muscle tissue stained for Carbonic Anhydrase 4/CA4 using ab239505 at 30 µg/ml in immunohistochemical analysis. DAB staining.
All lanes : Anti-Carbonic Anhydrase 4/CA4 antibody [C1] (ab239505) at 2 µg/ml
Lane 1 : Rat lung lysate
Lane 2 : Rat brain lysate
All lanes : HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution
Predicted band size: 35 kDa
Anti-Carbonic Anhydrase 4/CA4 antibody [C1] (ab239505) at 2 µg/ml + Recombinant rat Carbonic Anhydrase IV/CA4 protein
HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution
Predicted band size: 35 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab239505 has not yet been referenced specifically in any publications.