Anti-Carbonic Anhydrase 5A/CA5A antibody (ab231676)
Key features and details
- Rabbit polyclonal to Carbonic Anhydrase 5A/CA5A
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Carbonic Anhydrase 5A/CA5A antibody -
Description
Rabbit polyclonal to Carbonic Anhydrase 5A/CA5A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Recombinant fragment (His-tag) corresponding to Rat Carbonic Anhydrase 5A/CA5A aa 1-250. Expressed in E.coli. N-terminal tag.
Database link: P43165 -
Positive control
- WB: Recombinant rat Carbonic Anhydrase 5A/CA5A protein; rat serum; rat heart, lung and spleen lysates. IHC-P: Rat liver tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231676 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 35 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 35 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Relevance
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA5A is localized in the mitochondria and expressed primarily in the liver. It may play an important role in ureagenesis and gluconeogenesis. CA5A gene maps to chromosome 16q24.3 and an unprocessed pseudogene has been assigned to 16p12-p11.2. -
Cellular localization
Mitochondrial -
Database links
- Entrez Gene: 54233 Rat
- SwissProt: P43165 Rat
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Alternative names
- CA VA antibody
- CA5 antibody
- Carbonate dehydratase VA antibody
see all
Images
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All lanes : Anti-Carbonic Anhydrase 5A/CA5A antibody (ab231676) at 1.5 µg/ml
Lane 1 : Rat serum
Lane 2 : Rat heart lysate
Lane 3 : Rat lung lysate
Lane 4 : Rat spleen lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/1000 dilution
Predicted band size: 35 kDa -
Formalin-fixed, paraffin-embedded rat liver tissue stained for Carbonic Anhydrase 5A/CA5A using ab231676 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Anti-Carbonic Anhydrase 5A/CA5A antibody (ab231676) at 1.5 µg/ml + Recombinant rat Carbonic Anhydrase 5A/CA5A protein
Secondary
HRP-Linked Guinea pig anti-Rabbit
Predicted band size: 35 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231676 has not yet been referenced specifically in any publications.