Anti-Carbonic Anhydrase 5B/CA5B antibody (ab196800)
Key features and details
- Rabbit polyclonal to Carbonic Anhydrase 5B/CA5B
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Carbonic Anhydrase 5B/CA5B antibody
See all Carbonic Anhydrase 5B/CA5B primary antibodies -
Description
Rabbit polyclonal to Carbonic Anhydrase 5B/CA5B -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human Carbonic Anhydrase 5B/CA5B (internal sequence).
Database link: Q9Y2D0 -
Positive control
- NIH/3T3 cell extracts; MCF7 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/500.
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WB |
1/500 - 1/3000. Predicted molecular weight: 36 kDa.
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Notes |
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ICC/IF
1/100 - 1/500. |
WB
1/500 - 1/3000. Predicted molecular weight: 36 kDa. |
Target
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Function
Reversible hydration of carbon dioxide. -
Tissue specificity
Strongest expression in heart, pancreas, kidney, placenta, lung, and skeletal muscle. Not expressed in liver. -
Sequence similarities
Belongs to the alpha-carbonic anhydrase family.
Contains 1 alpha-carbonic anhydrase domain. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 11238 Human
- Entrez Gene: 56078 Mouse
- Entrez Gene: 302669 Rat
- Omim: 300230 Human
- SwissProt: Q9Y2D0 Human
- SwissProt: Q9QZA0 Mouse
- SwissProt: Q66HG6 Rat
- Unigene: 653287 Human
see all -
Alternative names
- CA VB antibody
- CA-VB antibody
- CA5B antibody
see all
Images
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All lanes : Anti-Carbonic Anhydrase 5B/CA5B antibody (ab196800) at 1/500 dilution
Lane 1 : NIH/3T3 cell extract
Lane 2 : NIH/3T3 cell extract with synthesized peptide
Predicted band size: 36 kDa -
Immunofluorescence analysis of MCF7 cells, labeling Carbonic Anhydrase 5B/CA5B with ab196800 at 1/100 dilution. The image on the right is treated with synthesized peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab196800 has not yet been referenced specifically in any publications.