Recombinant
RabMAb

Recombinant Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] (ab210406)

Overview

  • Product name

    Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)]
    See all Carbonic Anhydrase 6/CA6 primary antibodies
  • Description

    Rabbit monoclonal [EPR13954(2)] to Carbonic Anhydrase 6/CA6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    within Human Carbonic Anhydrase 6/CA6 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P23280

  • Positive control

    • WB: Human parotid gland lysate. IHC-P: Human parotid gland tissue.
  • General notes

     

     

    Previously labelled as CA6.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13954(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab210406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).

Target

  • Function

    Reversible hydration of carbon dioxide. Its role in saliva is unknown.
  • Tissue specificity

    Major constituent of saliva.
  • Sequence similarities

    Belongs to the alpha-carbonic anhydrase family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • CA VI antibody
    • CA-VI antibody
    • CA6 antibody
    • CAH6_HUMAN antibody
    • Carbonate dehydratase VI antibody
    • Carbonic anhydrase 6 antibody
    • Carbonic anhydrase VI antibody
    • carbonic anhydrase VI nirs variant 1 antibody
    • carbonic anhydrase VI nirs variant 3 antibody
    • GUSTIN antibody
    • MGC21256 antibody
    • Salivary carbonic anhydrase antibody
    • Secreted carbonic anhydrase antibody
    see all

Images

  • All lanes : Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] (ab210406) at 1/1000 dilution

    Lane 1 : Human parotid gland lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 35 kDa
    Observed band size: 35 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human parotid gland tissue labeling Carbonic Anhydrase 6/CA6 with ab210406 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on Human parotid gland is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Carbonic Anhydrase 6/CA6 with ab210406 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative staining on Human kidney.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab210406 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab210406.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up