Recombinant
RabMAb

Recombinant Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408)

Overview

  • Product name

    Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304]
    See all Carbonic Anhydrase 7/CA7 primary antibodies
  • Description

    Rabbit monoclonal [EPR13304] to Carbonic Anhydrase 7/CA7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    corresponding to Human Carbonic Anhydrase 7/CA7 aa 250 to the C-terminus.
    Database link: P43166

  • Positive control

    • Human fetal brain and fetal kidney tissue lysates; 293 and Raw264.7 cell lysates; HeLa cells.
  • General notes

     

     

     This product was previously labelled as CA7

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13304
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 30 kDa.
ICC/IF 1/50.
IP 1/20.

Target

Images

  • All lanes : Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408) at 1/5000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa

  • Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408) at 1/20000 dilution + Human fetal kidney lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa

  • Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408) at 1/5000 dilution + Raw264.7 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa

  • Immunofluorescence analysis of HeLa cells labeling Carbonic Anhydrase 7/CA7 using ab191408 at 1/50 dilution (4 μg/ml). A Goat anti rabbit IgG (Alexa Fluor488) at 1/200 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% Paraformaldehyde and permealized with 0.1% triton X-100. Counterstain: DAPI. The 2 negative cotrol images were obtained using Goat anti Mouse IgG (Alexa Fluor®594) at 1:400 dilution as secondary antibody.

  • Immunoprecipitation analysis of Human fetal brain tissue lysate labeling Carbonic Anhydrase 7/CA7 using ab191408 at 1/20 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of Human fetal brain tissue lysate.

References

ab191408 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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