Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464)

Overview

  • Product name

    Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal
    See all Carbonic Anhydrase 9/CA9 primary antibodies
  • Description

    Mouse monoclonal [10F7A8] to Carbonic Anhydrase 9/CA9 - N-terminal
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Carbonic Anhydrase 9/CA9 aa 37-186 (N terminal). Expressed in E.coli. Predicted MW of fragment is 42 kDa.
    Sequence:

    PQRL PRMQEDSPLG GGSSGEDDPL GEEDLPSEED SPREEDPPGE EDLPGEEDLP GEEDLPEVKP KSEEEGSLKL EDLPTVEAPG DPQEPQNNAH RDKEGDDQSH WRYGGDPPWP RVSPACAGRF QSPVDIRPQL AAFCPALRPL ELLGFQ


    Database link: Q16790

  • Positive control

    • Carbonic Anhydrase 9/CA9 recombinant protein, Carbonic Anhydrase IX (amino acids: 37-186)-hIgGFc transfected HEK293 cell lysate, A431 cell line lysate, SW620 cell line lysate, NTERA2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab181464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 50 kDa.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
  • Tissue specificity

    Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
  • Sequence similarities

    Belongs to the alpha-carbonic anhydrase family.
    Contains 1 alpha-carbonic anhydrase domain.
  • Post-translational
    modifications

    Asn-346 bears high-mannose type glycan structures.
  • Cellular localization

    Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CA-IX antibody
    • CA9 antibody
    • CAH9_HUMAN antibody
    • CAIX antibody
    • Carbonate dehydratase IX antibody
    • Carbonic anhydrase 9 antibody
    • Carbonic anhydrase IX antibody
    • Carbonic dehydratase antibody
    • G250 antibody
    • Membrane antigen MN antibody
    • MN antibody
    • P54/58N antibody
    • pMW1 antibody
    • RCC associated protein G250 antibody
    • RCC-associated antigen G250 antibody
    • Renal cell carcinoma-associated antigen G250 antibody
    see all

Images

  • All lanes : Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464) at 1/500 dilution

    Lane 1 : A431 cell line lysate
    Lane 2 : SW620 cell line lysate

    Predicted band size: 50 kDa

  • All lanes : Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464) at 1/500 dilution

    Lane 1 : HEK293 cell lysate.
    Lane 2 : Carbonic Anhydrase 9/CA9 (Amino acids: 37-186)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 50 kDa

  • Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464) at 1/500 dilution + Carbonic Anhydrase 9/CA9 recombinant protein

    Predicted band size: 50 kDa

  • Flow cytometric analysis of NTERA2 cells labeling Carbonic Anhydrase 9/CA9 with ab181464 at 1/200 dilution (green) and negative control (red).

References

ab181464 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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