Overview

  • Product name

    Anti-Carbonic Anhydrase 9/CA9 antibody [2D3]
    See all Carbonic Anhydrase 9/CA9 primary antibodies
  • Description

    Mouse monoclonal [2D3] to Carbonic Anhydrase 9/CA9
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-P, ICC/IF, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Carbonic Anhydrase 9/CA9.
    Database link: Q16790

  • Positive control

    • Hela and A549 cell lysate; lung and colonic tissues and NTERA-2 cells.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR311638-1 are from Tissue Culture Supernatant

Properties

Applications

Our Abpromise guarantee covers the use of ab107257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB 1/500 - 1/2000. Predicted molecular weight: 50 kDa.
IHC-P 1/200 - 1/1000.
ICC/IF 1/200 - 1/1000.
ELISA 1/10000.

Target

  • Function

    Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
  • Tissue specificity

    Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
  • Sequence similarities

    Belongs to the alpha-carbonic anhydrase family.
    Contains 1 alpha-carbonic anhydrase domain.
  • Post-translational
    modifications

    Asn-346 bears high-mannose type glycan structures.
  • Cellular localization

    Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CA-IX antibody
    • CA9 antibody
    • CAH9_HUMAN antibody
    • CAIX antibody
    • Carbonate dehydratase IX antibody
    • Carbonic anhydrase 9 antibody
    • Carbonic anhydrase IX antibody
    • Carbonic dehydratase antibody
    • G250 antibody
    • Membrane antigen MN antibody
    • MN antibody
    • P54/58N antibody
    • pMW1 antibody
    • RCC associated protein G250 antibody
    • RCC-associated antigen G250 antibody
    • Renal cell carcinoma-associated antigen G250 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded lung tissues (left) and colonic tissues (right) using 1/200 ab107257 with DAB staining.
  • All lanes : Anti-Carbonic Anhydrase 9/CA9 antibody [2D3] (ab107257) at 1/500 dilution

    Lane 1 : Hela Cell lysate
    Lane 2 : A549 Cell Lysate

    Predicted band size: 50 kDa

  • Flow cytometric analysis of NTERA-2 cells using Carbonic Anhydrase 9/CA9 mouse ab107257 in green and negative control in purple.

References

This product has been referenced in:

  • Yang WJ  et al. Overexpression of Tie2 is associated with poor prognosis in patients with gastric cancer. Oncol Lett 15:8027-8033 (2018). Read more (PubMed: 29849805) »
  • Sun W  et al. Interaction between von Hippel-Lindau Protein and Fatty Acid Synthase Modulates Hypoxia Target Gene Expression. Sci Rep 7:7190 (2017). Read more (PubMed: 28775317) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Gastric cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer, pH 9
Permeabilization
Yes - Triton 0.025%
Specification
Gastric cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 03 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma primary cells)
Loading amount
50 µg
Specification
Glioblastoma primary cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 21 2012

Answer

Thank you for contacting us.
I am sorry that the vial you received did not contain the full amount of the product and I apologize for the inconvenience. I have issued a free of charge replacement vial with the order number of 1112020.
Please do not hesitate to contact us if you need anything further.

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Answer

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Read More

Answer

Thank you for contacting us. Unfortunately we do not have any kit corresponding to the detection of the CAIX protein. However, I would suggest to develop an ELISA assay using the CAIX protein ab114200 that would be coated on the plate. A control anti-CAIX, such as ab15086, for which the concentration is known, may be used to create a standard curve and to quantify the amount of anti-CAIX antibodies contained in the mouse sera. ab15086 is a rabbit antibody. A mouse antibody would ideal, unfortunately, the only mouse anti-CAIX we have (ab107257) is not purified. It is an ascites surpernatant and the concentration has therefore not been determined. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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